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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-19427 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NSD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-19427 targets NSD3 in ICC/IF, IHC (P), and WB applications and shows reactivity with human samples. For IHC staining, heat mediated antigen retrieval is recommended. The PA5-19427 immunogen is synthetic peptide derived from residues 500 - 600 of Human NSD3. PA5-19427 detects NSD3 which has a predicted molecular weight of approximately 72.6 kDa and 161 kDa.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of HeLa cell extract using Product # PA5-19427, NSD3 primary antibody at a dilution of 1/500 (lane 1). Staining of 293 cell extract (lane 2). Staining of HeLa cell extract and blocking peptide (lane 3). Staining of 293 cell extract and blocking peptide (lane 4). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
