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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-28972 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, Raji, mouse brain. Predicted reactivity: Mouse (96%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.
Tran KA, Dillingham CM, Sridharan R
The EMBO journal 2019 Nov 15;38(22):e101681
The EMBO journal 2019 Nov 15;38(22):e101681
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSD3 using 30 µg of HeLa lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a NSD3 polyclonal antibody (Product # PA5-28972) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSD3 Polyclonal Antibody (Product # PA5-28972). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 5% SDS PAGE. NSD3 Polyclonal Antibody (Product # PA5-28972) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSD3 Polyclonal Antibody (Product # PA5-28972). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with NSD3 Polyclonal Antibody (Product # PA5-28972) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NSD3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NSD3 Polyclonal Antibody (Product # PA5-28972) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
