Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-28972 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, Raji, mouse brain. Predicted reactivity: Mouse (96%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity.
Tran KA, Dillingham CM, Sridharan R
The EMBO journal 2019 Nov 15;38(22):e101681
The EMBO journal 2019 Nov 15;38(22):e101681
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NSD3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: NSD3 Polyclonal Antibody (Product # PA5-28972) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.