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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- RQ6310 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ADAMTS5 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ADAMTS5 antibody is suitable for use in Western blot/Immunohistochemistry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ADAMTS5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) HepG2, 3) U-2 OS, 4) SW620, 5) ThP-1, 6) Raji, 7) SGC-7901, 8) rat liver, 9) rat testis, 10) mouse liver and 11) mouse testis lysate with ADAMTS5 antibody. Predicted molecular weight ~102 kDa, but the processed forms can be observed at approx. 50-102 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placental tissue with ADAMTS5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
