PAB28576

antibody from Abnova Corporation

Targeting: IFI16 IFNGIP1, PYHIN2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28576

Provider

Abnova Corporation

Product name

IFI16 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant IFI16.

Antigen sequence

KEKFTPKKIIAIANYVCRNGFLEVYPFTLVADVNA
DRNMEIPKGLIRSASVTPKINQLCSQTKGSFVNGV
FEVHKKNVRGEFTYYEIQDNTGKMEVVVHGRLTTI
NCEEGDKLKLTCFELAPKSGNTGELRSVIHSHIKV
IK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of HEK293T cell lysate using IFI16 polyclonal antibody (Cat # PAB28576).Lane 1: Negative control (vector only)Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of U-251 MG cell line with IFI16 polyclonal antibody (Cat # PAB28576) shows positivity in nucleus and nucleoli. Fixation/Permeabilization: PFA/Triton X-104

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skin with IFI16 polyclonal antibody (Cat # PAB28576) shows strong nuclear positivity in epidermal cells. Retrieval method: HIER pH6

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)