PA5-39180

antibody from Invitrogen Antibodies

Targeting: IFI16 IFNGIP1, PYHIN2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-39180

Provider

Invitrogen Antibodies

Product name

Anti-IFI16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA5-39180 detects IFI16 in WB application with human samples.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Priming and Activation of Inflammasome by Canarypox Virus Vector ALVAC via the cGAS/IFI16-STING-Type I IFN Pathway and AIM2 Sensor.

Liu F, Niu Q, Fan X, Liu C, Zhang J, Wei Z, Hou W, Kanneganti TD, Robb ML, Kim JH, Michael NL, Sun J, Soong L, Hu H
Journal of immunology (Baltimore, Md. : 1950) 2017 Nov 1;199(9):3293-3305

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IFI16 was performed by loading 30 µg of THP-1 lysate in reducing sample buffer onto a 4-15% Precast Protein Gels. Proteins were transferred to PVDF membrane and followed by blocking in TBST+5% non-fat milk for one hour at room temperature. IFI16 was detected at approximately 88 kDa using an IFI16 polyclonal antibody (Product # PA5-39180) at a dilution of 1:500 overnight at 4°C on a rocking platform, followed by a Rabbit IgG Horseradish Peroxidase-conjugated Antibody at a dilution of 1:1,000 for one hour. Chemiluminescent detection was performed using SuperSignal™ West Pico Chemiluminescent Substrate (Product # 34080) and ImageQuant LAS 4000 (GE, 28-9558-10).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IFI16 in extracts from HeLa cells and HepG2 cells using an IFI16 polyclonal antibody (Product # PA5-39180).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-IFI16 Polyclonal Antibody (Product # PA5-39180) and a band at 82 kDa corresponding to IFI16 was observed across the cell lines tested and the expression was increased or detected in HeLa and HUVEC upon IFN gamma and IFN beta treatments respectively. Modified whole cell extracts (1% SDS) (30ug lysate) of HeLa (Lane 1), HeLa treated with IFN gamma (15ng/ml for 16hr) (Lane 2), HUVEC (Lane 3), HUVEC treated with IFN beta (1000 IU/ml for 6hr) (Lane 4), Raji (Lane 5), Ramos (Lane 6), THP-1 (Lane 7), Jurkat (Lane 8) and Daudi (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).