Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-83118 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83118, RRID:AB_11041205
- Product name
- Rabbit Polyclonal IFI16 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human IFI16 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references NOD-like receptor signaling and inflammasome-related pathways are highlighted in psoriatic epidermis.
Tervaniemi MH, Katayama S, Skoog T, Siitonen HA, Vuola J, Nuutila K, Sormunen R, Johnsson A, Linnarsson S, Suomela S, Kankuri E, Kere J, Elomaa O
Scientific reports 2016 Mar 15;6:22745
Scientific reports 2016 Mar 15;6:22745
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFI16 Antibody [NBP1-83118] - Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-IFI16 antibody. Corresponding IFI16 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFI16 Antibody [NBP1-83118] - Analysis in control (vector only transfected HEK293T lysate) and IFI16 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFI16 Antibody [NBP1-83118] - Staining of human lymph node shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFI16 Antibody [NBP1-83118] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IFI16 Antibody [NBP1-83118] - Induced expression of NOD2, CARD6, PYCARD, and IFI16 in psoriasis lesions. Immunohistochemistry shows stronger NOD2, CARD6, PYCARD, and IFI16 staining in PL (upper) than in controls (lower). PN (middle) and C are almost negative for NOD2 and CARD6. IFI16 is predominantly in nuclei of PL and PN. In controls IFI16 expression is weak and cytoplasmic. PYCARD is strongly induced in cytoplasm but also some nuclei are positive in PL and PN. Controls exhibit only a few PYCARD positive nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep22745) licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFI16 Antibody [NBP1-83118] - Staining of human Fallopian tube shows moderate nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFI16 Antibody [NBP1-83118] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFI16 Antibody [NBP1-83118] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFI16 Antibody [NBP1-83118] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFI16 Antibody [NBP1-83118] - Staining in human lymph node and skeletal muscle tissues using NBP1-83118 antibody. Corresponding IFI16 RNA-seq data are presented for the same tissues.