H00009735-M01

antibody from Abnova Corporation

Targeting: KNTC1 KIAA0166, ROD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009735-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009735-M01, RRID:AB_464411

Product name

KNTC1 monoclonal antibody (M01), clone 10H4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant KNTC1.

Antigen sequence

DPQVILKQLEEHMNTGQLAGFSHQIRSLILNNIIN
KKEFGILAKTKYFQMLKMHAMNTNNITELVNYLAN
DLSLDEASVLITEYSKHCGKPVPPDTAPCEILKMF
LSGLS


Isotype

IgG

Antibody clone number

10H4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.

Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A
The Journal of cell biology 2009 Jun 1;185(5):859-74

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Modulations of cell cycle checkpoints during HCV associated disease.

Sarfraz S, Hamid S, Ali S, Jafri W, Siddiqui AA
BMC infectious diseases 2009 Aug 10;9:125

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

KNTC1 monoclonal antibody (M01), clone 10H4 Western Blot analysis of KNTC1 expression in Hela S3 NE ( Cat # L013V3 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged KNTC1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to KNTC1 on HeLa cell. [antibody concentration 50 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol