NB100-88131

antibody from Novus Biologicals

Targeting: KNTC1 KIAA0166, ROD

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-88131

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-88131, RRID:AB_1217828

Product name

Rabbit Polyclonal KNTC1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of Human ROD/KNTC1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 mcg/mg lysate. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds. (Lower bands that were immunoprecipitated by both NB100-88130 and NB100-88131 are marked by asterisks.)

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: KNTC1 Antibody [NB100-88131] - Detection of human ROD/KNTC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROD/KNTC1 antibody NB100-88131 used for IP at 3 ug per reaction. ROD/KNTC1 was also immunoprecipitated by rabbit anti-ROD/KNTC1 antibody NB100-88130. For blotting immunoprecipitated ROD/KNTC1, NB100-88131 was used at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.