Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF4666 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Endomucin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Endomucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Endomucin-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9R0H2
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome.
PECAM/eGFP transgenic mice for monitoring of angiogenesis in health and disease.
Endothelial cells are not required for specification of respiratory progenitors.
Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development.
Sox17 is required for normal pulmonary vascular morphogenesis.
Lamm KYB, Johnson ML, Baker Phillips J, Muntifering MB, James JM, Jones HN, Redline RW, Rokas A, Muglia LJ
eLife 2018 Jan 8;7
eLife 2018 Jan 8;7
PECAM/eGFP transgenic mice for monitoring of angiogenesis in health and disease.
Winkler F, Herz K, Rieck S, Kimura K, Hu T, Röll W, Hesse M, Fleischmann BK, Wenzel D
Scientific reports 2018 Dec 4;8(1):17582
Scientific reports 2018 Dec 4;8(1):17582
Endothelial cells are not required for specification of respiratory progenitors.
Havrilak JA, Melton KR, Shannon JM
Developmental biology 2017 Jul 1;427(1):93-105
Developmental biology 2017 Jul 1;427(1):93-105
Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development.
Ramo K, Sugamura K, Craige S, Keaney JF, Davis RJ
eLife 2016 Aug 9;5
eLife 2016 Aug 9;5
Sox17 is required for normal pulmonary vascular morphogenesis.
Lange AW, Haitchi HM, LeCras TD, Sridharan A, Xu Y, Wert SE, James J, Udell N, Thurner PJ, Whitsett JA
Developmental biology 2014 Mar 1;387(1):109-20
Developmental biology 2014 Mar 1;387(1):109-20
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Endomucin by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Endomucin in bEnd.3 Mouse Cell Line. Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Endomucin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).