ABIN359745

antibody from antibodies-online

Targeting: CUL5 VACM-1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN359745

Provider

antibodies-online

Product name

anti-Cullin 5 (CUL5) (C-Term) antibody

Antibody type

Polyclonal

Description

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reactivity

Human

Host

Rabbit

Epitope

C-Term

Vial size

0.4 mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases.

Kamura T, Maenaka K, Kotoshiba S, Matsumoto M, Kohda D, Conaway RC, Conaway JW, Nakayama KI
Genes & development 2004 Dec 15;18(24):3055-65

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Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation.

Mehle A, Goncalves J, Santa-Marta M, McPike M, Gabuzda D
Genes & development 2004 Dec 1;18(23):2861-6

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Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase.

Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW
The Journal of biological chemistry 2001 Aug 10;276(32):29748-53

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB