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- Antigen structure
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- Immunohistochemistry [1]
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- Product number
- PA5-14470 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIPK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis.
Li X, Qu M, Zhang J, Chen K, Ma X
Journal of orthopaedic surgery and research 2021 May 8;16(1):302
Journal of orthopaedic surgery and research 2021 May 8;16(1):302
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HIPK2 in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with HIPK2 polyclonal antibody (Product # PA5-14470) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 CircASH2L indirectly regulates HIPK2 via miR-129-5p in RA-FLSs. a Binding sites of miR-129-5p on HIPK2 3''UTR. b Interaction analysis between miR-129-5p and HIPK2 using the dual-luciferase reporter assay. c - f HIPK2 expression level in synovial tissues with RA and non-RA, as well as in RA-FLSs and N-FLSs was determined. g MiR-129-5p expression analysis using qRT-PCR in RA-FLSs transfected with miR-NC or miR-129-5p. h , i HIPK2 expression analysis in miR-129-5p-increased RA-FLSs with qRT-PCR and western blot. j , k RA-FLSs were co-transfected with si-circASH2L and/or anti-miR-129-5p and HIPK2 level was examined using qRT-PCR and western blot. * P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 MiR-129-5p impairs RA-FLS tumor-like biologic behaviors through HIPK2. The miR-129-5p and/or HIPK2 were co-transfected into RA-FLSs. After transfection, a western blot analysis of HIPK2 expression using western blot; b , c MTT assay of cell proliferation and Edu assay; d flow cytometry of cell apoptosis detection; e western blot analysis of apoptosis-related protein expression in cells; f flow cytometry for cell cycle analysis; g , h cell migratory and invasive abilities detection using transwell assay; i the measurement of inflammatory cytokines levels using ELISA. * P
