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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-80883 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PARVA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human Actopaxin.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PARVA Polyclonal Antibody (Product # PA5-80883) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: A549 Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 48 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PARVA Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with PARVA Polyclonal Antibody (Product # PA5-80883) and 15 µL of 50 % Protein G agarose. Primary antibody: PARVA Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PARVA Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with PARVA Polyclonal Antibody (Product # PA5-80883) and 15 µL of 50 % Protein G agarose. Primary antibody: PARVA Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa.
