MA5-15433

antibody from Invitrogen Antibodies

Targeting: FABP4 A-FABP, aP2

ELISA

Antibody data

Submit

Validation data

Product number: MA5-15433 - Provider product page
Provider: Invitrogen Antibodies
Product name: FABP4 Monoclonal Antibody (5H11A4E11)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15433 targets FABP4 in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15433 immunogen is purified recombinant fragment of FABP4 (aa61-121) expressed in E. Coli.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 5H11A4E11
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

FABP4 protein structure - MA5-15433 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FABP4 using a FABP4 monoclonal antibody (Product # MA5-15433) against a truncated Trx-FABP4 recombinant protein (1).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Fatty acid-binding protein, adipocyte was achieved by transfecting 3T3-L1 cells with Fatty acid-binding protein, adipocyte specific siRNAs (Silencer® select Product # s62398, s62400) and then the cells were differentiated to adipocytes. Western blot analysis (Fig. a) was performed using Whole cell extracts from the Fatty acid-binding protein, adipocyte knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FABP4 Monoclonal Antibody (5H11A4E11) (Product # MA5-15433, 1:1,000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fatty acid-binding protein, adipocyte.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Fatty acid-binding protein, adipocyte was achieved by transfecting differentiated 3T3-L1 cells to adipocytes, with Fatty acid-binding protein, adipocyte specific siRNAs (Silencer® select Product # s62398, s62400). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Fatty acid-binding protein, adipocyte knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FABP4 Monoclonal Antibody (5H11A4E11) (Product # MA5-15433, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fatty acid-binding protein, adipocyte.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-FABP4 Monoclonal Antibody (5H11A4E11) (Product # MA5-15433) and a 15 kDa band corresponding to Fatty acid-binding protein, adipocyte was observed across cell lines. Whole cell extracts (40 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Upon differentiation of 3T3-L1 cells to adipocytes the expression of FABP4 should upregulate, which was observed here along with a non specific band.