MA5-15433

antibody from Invitrogen Antibodies

Targeting: FABP4 A-FABP, aP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15433

Provider

Invitrogen Antibodies

Product name

FABP4 Monoclonal Antibody (5H11A4E11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15433 targets FABP4 in WB applications and shows reactivity with Human samples.

Antibody clone number

5H11A4E11

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FABP4 using a FABP4 monoclonal antibody (Product # MA5-15433) against a truncated Trx-FABP4 recombinant protein (1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of Fatty acid-binding protein, adipocyte was achieved by transfecting 3T3-L1 cells with Fatty acid-binding protein, adipocyte specific siRNAs (Silencer® select Product # s62398, s62400) and then the cells were differentiated to adipocytes. Western blot analysis (Fig. a) was performed using Whole cell extracts from the Fatty acid-binding protein, adipocyte knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FABP4 Monoclonal Antibody (5H11A4E11) (Product # MA5-15433, 1:1,000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fatty acid-binding protein, adipocyte.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of Fatty acid-binding protein, adipocyte was achieved by transfecting differentiated 3T3-L1 cells to adipocytes, with Fatty acid-binding protein, adipocyte specific siRNAs (Silencer® select Product # s62398, s62400). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Fatty acid-binding protein, adipocyte knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FABP4 Monoclonal Antibody (5H11A4E11) (Product # MA5-15433, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fatty acid-binding protein, adipocyte.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-FABP4 Monoclonal Antibody (5H11A4E11) (Product # MA5-15433) and a 15 kDa band corresponding to Fatty acid-binding protein, adipocyte was observed across cell lines. Whole cell extracts (40 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). Upon differentiation of 3T3-L1 cells to adipocytes the expression of FABP4 should upregulate, which was observed here along with a non specific band.