MA5-15566

antibody from Invitrogen Antibodies

Targeting: FABP4 A-FABP, aP2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15566

Provider

Invitrogen Antibodies

Product name

FABP4 Monoclonal Antibody (9B8D)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15566 targets FABP4 in WB applications and shows reactivity with Human samples.

Antibody clone number

9B8D

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FABP4 using FABP4 monoclonal antibody (Product # MA5-15566) in FABP4-human IgG Fc transfected HEK293 cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FABP4 using FABP4 monoclonal antibody (Product # MA5-15566) in FABP4-human IgG Fc transfected HEK293 cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FABP4 using FABP4 monoclonal antibody (Product # MA5-15566) in FABP4-human IgG Fc transfected HEK293 cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-FABP4 Monoclonal Antibody (9B8D) (Product # MA5-15566) and a 15 kDa band corresponding to Fatty acid-binding protein, adipocyte was observed across cell lines and tissues. Whole cell extracts (40 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), Mouse Adipose (Lane 3), Rat Adipose (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). Upon differentiation of 3T3-L1 cells to adipocytes the expression of FABP4 should upregulate, as observed and also, relative expression was observed in adipose over brain, as reported.