- PA5-30591 - Invitrogen Antibodies FABP4 antibody

Submitted references Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis.

Wu J, Song D, Li Z, Guo B, Xiao Y, Liu W, Liang L, Feng C, Gao T, Chen Y, Li Y, Wang Z, Wen J, Yang S, Liu P, Wang L, Wang Y, Peng L, Stacey GN, Hu Z, Feng G, Li W, Huo Y, Jin R, Shyh-Chang N, Zhou Q, Wang L, Hu B, Dai H, Hao J
Cell research 2020 Sep;30(9):794-809

In vitro osteoblastic differentiation of mesenchymal stem cells generates cell layers with distinct properties.

Hanna H, Mir LM, Andre FM
Stem cell research & therapy 2018 Jul 27;9(1):203

A fully defined static suspension culture system for large-scale human embryonic stem cell production.

Li X, Ma R, Gu Q, Liang L, Wang L, Zhang Y, Wang X, Liu X, Li Z, Fang J, Wu J, Wang Y, Li W, Hu B, Wang L, Zhou Q, Hao J
Cell death & disease 2018 Aug 30;9(9):892

Heterogeneous Differentiation of Human Mesenchymal Stem Cells in 3D Extracellular Matrix Composites.

Jung JP, Bache-Wiig MK, Provenzano PP, Ogle BM
BioResearch open access 2016;5(1):37-48

Tube formation in the first trimester placental trophoblast cells: Differential effects of angiogenic growth factors and fatty acids.

Pandya AD, Das MK, Sarkar A, Vilasagaram S, Basak S, Duttaroy AK
Cell biology international 2016 Jun;40(6):652-61

Age-dependent changes in TDP-43 levels in a mouse model of Alzheimer disease are linked to Aβ oligomers accumulation.

Caccamo A, Magrí A, Oddo S
Molecular neurodegeneration 2010 Nov 11;5:51

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FABP4 using 30 µg of 293T lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a FABP4 polyclonal antibody (Product # PA5-30591) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of Fatty acid-binding protein, adipocyte was achieved by transfecting 3T3-L1 cells with Fatty acid-binding protein, adipocyte specific siRNAs (Silencer® select Product # s62398, s62400) and then the cells were differentiated to adipocytes. Western blot analysis (Fig. a) was performed using Whole cell extracts from the Fatty acid-binding protein, adipocyte knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FABP4 Polyclonal Antibody (Product # PA5-30591, 1:2,000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fatty acid-binding protein, adipocyte. A non-specific band was observed at 30 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using FABP4 Polyclonal Antibody (Product # PA5-30591). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 Polyclonal Antibody (Product # PA5-30591) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Fatty acid-binding protein, adipocyte was achieved by transfecting differentiated 3T3-L1 cells to adipocytes with Fatty acid-binding protein, adipocyte specific siRNAs (Silencer® select Product # s62398, s62400). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Fatty acid-binding protein, adipocyte knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FABP4 Polyclonal Antibody (Product # PA5-30591, 1:2,000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Fatty acid-binding protein, adipocyte. A non specific band was observed at 30 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-FABP4 Polyclonal Antibody (Product # PA5-30591) and a 15 kDa band corresponding to Fatty acid-binding protein, adipocyte was observed across cell lines. Whole cell extracts (40 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Upon differentiation of 3T3-L1 cells to adipocytes the expression of FABP4 should upregulate as observed along with a non specific band at ~ 30 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of FABP4 in paraformaldehyde-fixed mouse ESC D3 cells using a FABP4 polyclonal antibody (Product # PA5-30591) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of FABP4 in paraformaldehyde-fixed HeLa cells using a FABP4 polyclonal antibody (Product # PA5-30591) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIG. 4. Expression of FABP4 and PPAR-gamma proteins in 3D ColI composites after 28 days of culture. Immunofluorescence was visualized by MPLSM. FABP4 (a-c) and PPAR-gamma (d-f) . Adipogenic protein markers (green) and nuclei (blue); scale bar 50 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Comparison of MSCs derived from 2D-hESCs and 3D-hESCs. a The morphology of 2D- and 3D-hESC-MSCs at different stages of differentiation. b Flow cytometry analysis revealed specific MSC surface markers (CD44, CD29, and CD105) with negative controls (CD34, CD19, and CD45) in 2D- and 3D-hESC-MSCs. c Immunostaining of differentiated 3D-hESC-MSCs expressing an adipocyte marker (FABP-4), osteocytes maker (osteocalcin), and chondrocytes marker (aggrecan)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 TDP-43 and TDP-35 levels do not correlate with tau phosphorylated at Thr181 . (A) Representative microphotographs of 3xTg-AD brain sections depicting hippocampal CA1 pyramidal neurons stained with the anti-tau antibody AT270, which recognize tau phosphorylated at Thr181 (n = 6/age group). (B) Representative Western blots of proteins extracted from 6- and 12-month old 3xTg-AD mice probed with the AT270 antibody. beta-actin is used as a loading control. (C) Quantitative analysis of the blots shows that the steady-state levels of AT270 were significantly higher in the brains of 12-month-old 3xTg-AD mice compared to 6-month-old mice (n = 6/age group; p < 0.05). Data are presented as +- SEM and analyzed by t-test analysis. (D-E) Linear regression analysis indicated that the levels of AT270 in the brains of 6-month-old 3xTg-AD mice did not correlate with the levels of TDP-43 (r 2 = 0.09148, p = 0.56) and TDP-35 (r 2 = 0.1491, p = 0.45). (F-G) Similarly, no correlation between AT270 and TDP-43 (r 2 = 0.4644, p = 0.14) and TDP-35 (r 2 = 0.4469, p = 0.15) levels was observed at 12 months of age.

PA5-30591

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