Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-105918 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SIK1 (Thr182) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of SIK only when phosphorylated at Thr182.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Role of SIK1 in the transition of acute kidney injury into chronic kidney disease.
Hu J, Qiao J, Yu Q, Liu B, Zhen J, Liu Y, Ma Q, Li Y, Wang Q, Wang C, Lv Z
Journal of translational medicine 2021 Feb 15;19(1):69
Journal of translational medicine 2021 Feb 15;19(1):69
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SIK1 (Thr182) in EGF treated HeLa cell lysate (absence (-) or presence (+) of non-phospho and phospho peptide). Samples were incubated with Phospho-SIK1 (Thr182) polyclonal antibody (Product # PA5-105918).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SIK1 (Thr182) in HeLa cell lysate. Samples were incubated with Phospho-SIK1 (Thr182) polyclonal antibody (Product # PA5-105918).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-SIK1 (Thr182) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SIK1 (Thr182) polyclonal antibody (Product # PA5-105918) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-SIK1 (Thr182) in mouse testis tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SIK1 (Thr182) polyclonal antibody (Product # PA5-105918) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded Phospho-SIK1 (Thr182) in rat liver tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SIK1 (Thr182) polyclonal antibody (Product # PA5-105918) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.