18304-1-AP

antibody from Proteintech Group

Targeting: THBS1 THBS, THBS-1, TSP, TSP-1, TSP1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

18304-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18304-1-AP, RRID:AB_2201959

Product name

Thrombospondin 1 antibody

Antibody type

Polyclonal

Description

KD/KO validated Thrombospondin 1 antibody (Cat. #18304-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

THBS1 promotes angiogenesis and accelerates ESCC malignant progression by the HIF-1/VEGF signaling pathway.

Zhou X, Xia Q, Chen M, Zhang X, Huang M, Zheng X, Wang S, Wu B, Du Z
Cell biology international 2024 Mar;48(3):311-324

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Ntsr1 contributes to pulmonary hypertension by enhancing endoplasmic reticulum stress via JAK2-STAT3-Thbs1 signaling.

Wei ZX, Cai XX, Fei YD, Wang Q, Hu XL, Li C, Hou JW, Yang YL, Wang YP, Li YG
Translational research : the journal of laboratory and clinical medicine 2024 Jul;269:64-75

---

Targeting Thbs1 reduces bladder remodeling caused by partial bladder outlet obstruction via the FGFR3/p-FGFR3 pathway.

Long J, Yang Y, Yang J, Chen L, Wang S, Zhou X, Su Y, Liu C
Neurourology and urodynamics 2024 Feb;43(2):516-526

---

Exploration and validation of signature genes and immune associations in septic cardiomyopathy.

Zhao Z, Wang X, Tan F, Liu H, Chen W, Wang J, Deng S, Du J
Clinical and experimental hypertension (New York, N.Y. : 1993) 2024 Dec 31;46(1):2302570

---

Integrated transcriptomic and proteomic profiling reveals the key molecular signatures of brain endothelial reperfusion injury.

Ji Y, Chen Y, Tan X, Huang X, Gao Q, Ma Y, Yang S, Yin M, Yu M, Fang C, Wang Y, Shi Z, Chang J
CNS neuroscience & therapeutics 2024 Apr;30(4):e14483

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hsa_circ_0007919 promotes pancreatic cancer metastasis by modulating Sp1-mediated THBS1 transcription.

Ma X, Xu L, Gong S, Wu N, Guo J, Feng X, Zhao M, Qiu S, Sun M, Zhang C, Zhang X, Ren Z, Zhang P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Apr 15;38(7):e23591

---

Transplantation of Human Amniotic Mesenchymal Stem Cells Up-Regulates Angiogenic Factor Expression to Attenuate Diabetic Kidney Disease in Rats.

Ni Y, Chen Y, Jiang X, Pu T, Zhang L, Li S, Hu L, Bai B, Hu T, Yu L, Yang Y
Diabetes, metabolic syndrome and obesity : targets and therapy 2023;16:331-343

---

FN1 mRNA 3'-UTR supersedes traditional fibronectin 1 in facilitating the invasion and metastasis of gastric cancer through the FN1 3'-UTR-let-7i-5p-THBS1 axis.

Pan S, Zhu J, Liu P, Wei Q, Zhang S, An W, Tong Y, Cheng Z, Liu F
Theranostics 2023;13(14):5130-5150

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Transcriptomic characterization revealed that METTL7A inhibits melanoma progression via the p53 signaling pathway and immunomodulatory pathway.

Zhang D, Zou T, Liu Q, Chen J, Xiao M, Zheng A, Zhang Z, Du F, Dai Y, Xiang S, Wu X, Li M, Chen Y, Zhao Y, Shen J, Chen G, Xiao Z
PeerJ 2023;11:e15799

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Exosomes derived from menstrual blood stromal cells ameliorated premature ovarian insufficiency and granulosa cell apoptosis by regulating SMAD3/AKT/MDM2/P53 pathway via delivery of thrombospondin-1.

Song A, Zhang S, Zhao X, Wu S, Qi X, Gao S, Qi J, Li P, Tan J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Oct;166:115319

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Investigation of a UPR-Related Gene Signature Identifies the Pro-Fibrotic Effects of Thrombospondin-1 by Activating CD47/ROS/Endoplasmic Reticulum Stress Pathway in Lung Fibroblasts.

Zhan JH, Wei J, Liu L, Xu YT, Ji H, Wang CN, Liu YJ, Zhu XY
Antioxidants (Basel, Switzerland) 2023 Nov 21;12(12)

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Gastric cancer derived exosomal THBS1 enhanced Vγ9Vδ2 T-cell function through activating RIG-I-like receptor signaling pathway in a N6-methyladenosine methylation dependent manner.

Li J, Feng H, Zhu J, Yang K, Zhang G, Gu Y, Shi T, Chen W
Cancer letters 2023 Nov 1;576:216410

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Deletion of Bak1 alleviates microglial necroptosis and neuroinflammation after experimental subarachnoid hemorrhage.

Qiu X, Tao Q, Zhang L, Kuang C, Xie Y, Zhang L, Yin S, Peng J, Jiang Y
Journal of neurochemistry 2023 Mar;164(6):829-846

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MLL4 Regulates the Progression of Non-Small-Cell Lung Cancer by Regulating the PI3K/AKT/SOX2 Axis.

Yang Y, Qiu R, Weng Q, Xu Z, Song J, Zhao S, Meng M, Zhang D, Kong C, Wang H, Xu M, Zhao Z, Ji J
Cancer research and treatment 2023 Jul;55(3):778-803

---

CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis.

Singla B, Aithbathula RV, Pervaiz N, Kathuria I, Swanson M, Ekuban FA, Ahn W, Park F, Gyamfi M, Cherian-Shaw M, Singh UP, Kumar S
Arteriosclerosis, thrombosis, and vascular biology 2023 Jul;43(7):1234-1250

---

Hybrids of carbonic anhydrase and cyclooxygenase inhibitors attenuate cardiac hypoxic inflammatory injuries.

Zhou W, Wang C, Zhang B, Gou S
European journal of pharmacology 2023 Jul 5;950:175751

---

Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos.

Zhu Q, Tan M, Wang C, Chen Y, Wang C, Zhang J, Gu Y, Guo Y, Han J, Li L, Jiang R, Fan X, Xie H, Wang L, Gu Z, Liu D, Shi J, Feng X
Cell & bioscience 2023 Jul 19;13(1):130

---

PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivation.

Peng Z, Tan X, Xie L, Li Z, Zhou S, Li Y
iScience 2023 Jan 20;26(1):105909

---

Yth m(6)A RNA-Binding Protein 1 Regulates Osteogenesis of MC3T3-E1 Cells under Hypoxia via Translational Control of Thrombospondin-1.

Shi D, Liu X, Li X, Li T, Liu J, Wu L
International journal of molecular sciences 2023 Jan 16;24(2)

---

Discovery of Efficient Hypoxia-Targeted NO Donor Compounds to Alleviate Hypoxia Cardiac Disease.

Yang W, Zhou W, Gou S
Journal of medicinal chemistry 2023 Dec 14;66(23):15977-15989

---

Lactic acidosis induces metabolic and phenotypic reprogramming in cholangiocarcinoma cells via the upregulation of thrombospondin-1.

Thamrongwaranggoon U, Kuribayashi K, Araki H, Hino Y, Koga T, Seubwai W, Wongkham S, Nakao M, Hino S
Cancer science 2023 Apr;114(4):1541-1555

---

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Liu X, Li N, Zhang C, Wu X, Zhang S, Dong G, Liu G
Translational oncology 2022 May;19:101389

---

Circ_0047339 promotes the activation of fibroblasts and affects the development of urethral stricture by targeting the miR-4691-5p/TSP-1 axis.

Ding K, Li D, Zhang R, Zuo M
Scientific reports 2022 Aug 30;12(1):14746

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miR-19a-3p Promotes Tumor-Relevant Behaviors in Bladder Urothelial Carcinoma via Targeting THBS1.

Xu G, Li J, Yu L
Computational and mathematical methods in medicine 2021;2021:2710231

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MiR-18a-5p acts as a novel serum biomarker for venous malformation and promotes angiogenesis by regulating the thrombospondin-1/P53 signaling axis.

Zhang L, Wang D, Wang Z, Li X, Xia W, Han Y, Su L, Fan X
American journal of translational research 2021;13(10):11271-11286

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tRNA-Derived Fragment tRF-17-79MP9PP Attenuates Cell Invasion and Migration via THBS1/TGF-β1/Smad3 Axis in Breast Cancer.

Mo D, He F, Zheng J, Chen H, Tang L, Yan F
Frontiers in oncology 2021;11:656078

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Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study.

Guo H, Li H, Feng Y, Ke J, Fang W, Li C, Long X
Oral surgery, oral medicine, oral pathology and oral radiology 2021 May;131(5):558-564

---

Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.

Xiao Y, Cong M, Li J, He D, Wu Q, Tian P, Wang Y, Yang S, Liang C, Liang Y, Wen J, Liu Y, Luo W, Lv X, He Y, Cheng DD, Zhou T, Zhao W, Zhang P, Zhang X, Xiao Y, Qian Y, Wang H, Gao Q, Yang QC, Yang Q, Hu G
Cancer cell 2021 Mar 8;39(3):423-437.e7

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The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.

Nording H, Baron L, Haberthür D, Emschermann F, Mezger M, Sauter M, Sauter R, Patzelt J, Knoepp K, Nording A, Meusel M, Meyer-Saraei R, Hlushchuk R, Sedding D, Borst O, Eitel I, Karsten CM, Feil R, Pichler B, Erdmann J, Verschoor A, Chavakis E, Chavakis T, von Hundelshausen P, Köhl J, Gawaz M, Langer HF
Nature communications 2021 Jun 7;12(1):3352

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High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation.

Bonnet-Magnaval F, Diallo LH, Brunchault V, Laugero N, Morfoisse F, David F, Roussel E, Nougue M, Zamora A, Marchaud E, Tatin F, Prats AC, Garmy-Susini B, DesGroseillers L, Lacazette E
International journal of molecular sciences 2021 Dec 25;23(1)

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The protective effect of HOXA5 on carotid atherosclerosis occurs by modulating the vascular smooth muscle cell phenotype.

Jing Y, Gao B, Han Z, Xia L, Xin S
Molecular and cellular endocrinology 2021 Aug 20;534:111366

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Serum Exosomal Proteins F9 and TSP-1 as Potential Diagnostic Biomarkers for Newly Diagnosed Epilepsy.

Lin Z, Gu Y, Zhou R, Wang M, Guo Y, Chen Y, Ma J, Xiao F, Wang X, Tian X
Frontiers in neuroscience 2020;14:737

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Discovery of small extracellular vesicle proteins from human serum for liver cirrhosis and liver cancer.

Uzzaman A, Zhang X, Qiao Z, Zhan H, Sohail A, Wahid A, Shang Z, Guan X, Cao CX, Xiao H
Biochimie 2020 Oct;177:132-141

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Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.

Zhao J, Meng M, Zhang J, Li L, Zhu X, Zhang L, Wang C, Gao M
Molecular medicine reports 2019 Apr;19(4):3168-3178

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Thrombospondin-1 Production Regulates the Inflammatory Cytokine Secretion in THP-1 Cells Through NF-κB Signaling Pathway.

Xing T, Wang Y, Ding WJ, Li YL, Hu XD, Wang C, Ding A, Shen JL
Inflammation 2017 Oct;40(5):1606-1621

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Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction.

Tang J, Shen Y, Chen G, Wan Q, Wang K, Zhang J, Qin J, Liu G, Zuo S, Tao B, Yu Y, Wang J, Lazarus M, Yu Y
Nature communications 2017 Mar 3;8:14656

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IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway.

Xu J, Cai H, Meng Q, Li Y, Chen G, Fang W, Long X
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2016 Sep;45(8):605-12

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Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia.

Guo H, Fang W, Chen G, Xu J, Li C, Feng Y, Li Y, Long X
Oral surgery, oral medicine, oral pathology and oral radiology 2016 Apr;121(4):e65-71

---

Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay.

Jia K, Li W, Wang F, Qu H, Qiao Y, Zhou L, Sun Y, Ma Q, Zhao X
Oncotarget 2016 Apr 26;7(17):23569-80

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP(TSP1 antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The Thrombospondin-1 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human Thrombospondin-1. This antibody recognizes human antigen. The Thrombospondin-1 antibody has been validated for the following applications: ELISA, WB, IF analysis.

Appendix

18304-1-AP.pdf

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