MA5-13395

antibody from Invitrogen Antibodies

Targeting: THBS1 THBS, THBS-1, TSP, TSP-1, TSP1

Provider product page for MA5-13395

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [120]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]
Other assay [8]

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Product number: MA5-13395 - Provider product page
Provider: Invitrogen Antibodies
Product name: Thrombospondin 1 Monoclonal Antibody (A6.1), Biotin
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-13395 targets Thrombospondin in FACS, IF, IHC (P), IM, IP, and WB applications and shows reactivity with Bovine, Canine, Equine, Human, mouse, Ovine, Porcine, and Rat samples.
Conjugate: Biotin
Antibody clone number: A6.1
Concentration: 0.2 mg/mL

THBS1 protein structure - MA5-13395 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Thrombospondin using Thrombospondin Monoclonal Antibody (Product # MA5-13395) on Native Human HUVEC Cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Thbs1 binds and activates a PERK-mediated ER stress response. a Representative western blots for ATF6alpha-N (50 kDa, nuclear), phospho-IRE1alpha (Ser 724; arrowhead), total IRE1alpha, phospho- and total PERK, phospho-eIF2alpha (Ser 51), total-eIF2alpha, ATF4, and DNAJC3. Phospho-PERK was analyzed using Phos-tag gels. The heart protein extracts are from the indicated mice at 4 weeks of age. Gapdh serves as a processing and loading control. b , c Western blot for Thbs1 and PERK following immunoprecipitation (IP) of PERK ( b ) or Thbs1 ( c ) from protein extracts of tTA cont. and Thbs1 DTG hearts at 6 weeks of age. IPs with corresponding IgG served as negative controls. Vinculin is an input loading control. d Schematic diagram of Thbs1 with the GST-Thbs1 fusion proteins regions shown (red bars). Below the schematic a representative western blot for PERK following GST pull-down with the different Thbs1 domains from primary neonatal rat ventricular cardiomyocyte extracts. GST protein serves as a negative control. e - i , Quantitative RT-PCR for Eif2ak3 (PERK protein; * P = 0.0317 vs tTA control), Atf4 (* P = 0.0006 vs tTA control), Atf3 (* P = 0.0058 vs tTA control), Ddit3 (Chop protein; * P < 0.0001 vs tTA control)) and Fgf21 (* P = 0.0023 vs tTA control) from mRNA isolated from hearts of tTA cont. (n = 6 biologically independent animals) and Thbs1 DTG mice (n = 5 biologically independent animals) at 6 weeks of age. Statistical analysis was performed using two-tailed Stud
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5--figure supplement 1. Patchy TSP1 immunoreactivity is endothelial and excluded from thoracic branch point orifices. ( A ) Whole-mount en face preparation of a mouse thoracic aorta immunostained for ITGA6, TSP1, and VEC. Scale bar is 200 uM. ( B ) Two sagittal sections ( b' and b'' ) of a mouse thoracic aorta demonstrate TSP1 expression in VEC+ arterial ECs. Sections were counterstained with DAPI to image nuclei. Scale bars are 20 uM.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Identification of aEC1 as arterial ECs around the circumference of branch point orifices. ( A ) Barplots of scRNA-seq read counts for Nog , Itga6 , Alpl , Cldn5 , S100a4 , Thbs1 , and Vcam1 in arterial EC clusters (aEC1-6). Each bar represents a single cell, either GFP high (green) or GFP low (blue). ( B-H ) Confocal images of whole-mount en face preparations of mouse thoracic aortae centered on intercostal branch points. Immunostaining of S1PR1-GS mouse aortae for noggin (NOG) and CD31 ( B ), or integrin alpha-6 (ITGA6) and VE-Cadherin (VEC) ( C ). Immunostaining of C57BL/6J mouse aortae for CD31 and alkaline phosphatase, tissue-nonspecific isozyme (ALPL) ( D ) or claudin-5 (CLDN5), FSP1 (S100A4), and VEC ( E ). ( F ) Immunostaining of S1PR1-GS mouse aorta for ITGA6, thrombospondin 1 (TSP1), and VEC. Yellow arrows indicate TSP1+ ECs. ( G ) Immunostaining of an S1PR1-GS mouse aorta for FSP1 and TSP1. The circumference of an intercostal branch point harbors FSP1+GFP+ cells, wereas TSP1+GFP+ cells (cyan arrows) are distal from the circumference of the branch point orifice. ( H ) Immunostaining of a mouse aorta for vascular cell adhesion molecule 1 (VCAM1) and VEC. Two rows of cells exhibiting the morphology and VEC-localization of cells around branch point orifices are outlined. Images are representative of observations from 3 ( B, C, E-H ) and 2 ( D ) mice. Scale bars are 50 uM. Figure 5--figure supplement 1. Patchy TSP1 immunoreactivity is endothelial and excluded f
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7--figure supplement 3. Embryonic expression of ITGA6, TSP1, and aEC1-enriched and -depleted transcripts. ( A ) Aggregated E9.5 to E13.5 scRNA-seq data from Cao et al. (2019) is shown for Itga6 and Thbs1 . Red arrows indicate normalized gene expression in endothelial cells. ( B-D ) Sagittal cryosections (14 uM) of an E11.5 S1PR1-GS mouse embryo immunostained for CD31, TSP1 and ITGA6 and counterstained with DAPI, as labeled on each panel. The dorsal aorta, lung bud, and hepatic primordium are indicated in ( B ). ( C ) The boxed region of the dorsal aorta is shown at higher magnification with CD31, ITGA6, and GFP channels. Scale bars are 200 uM. ( D ) High power (40x) image of the dorsal aorta. Scale bar is 20 uM. ( E ) Expression of selected aEC1-enriched and -depleted transcripts in aggregated E9.5-E13.5 mouse embryonic endothelial cells (data from Cao et al., 2019 ).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7. Spatio-temporal and molecular differences between branch point-specific arterial EC cluster aEC1 and non-branch point ECs. ( A ) Heatmap of selected transcription factors enriched and depleted in aEC1 cells (see also Figure 7--figure supplement 1A ). ( B ) Immunostaining of an S1PR1-GS mouse thoracic aorta for lymphoid enhancer-binding factor 1 (LEF1), ITGA6, and VEC. Image is representative of observations from two mice. Scale bar is 100 uM. ( C ) Immunostaining of postnatal day 6 ( P6 ) (n = 2) and 12 week (n = 3) S1PR1-GS mice thoracic aortae for ITGA6 and VEC. Arrows indicate non-branch point GFP+ EC. Scale bars are 100 uM. ( D ) Quantification of non-branch point GFP+ EC in thoracic aorta en face preparations from P6 (n = 2), P12 (n = 3), 3-4 month-old (n = 3), and 15 month old (n = 2) mice. Each dot is a field. Representative fields are shown in Figure 7--figure supplement 2 . Non-branch point GFP pixels were normalized to total VEC pixels per field. One-way ANOVA followed by Bonferroni's post hoc test, * P = 0.04; ** P = 0.04; *** P = 0.0008. ( E ) Representative images of TSP1 and VEC immunostaining of thoracic aorta en face preparations from P6, 3 month old, 15 month old mice. Scale bars are 200 uM. ( F ) Quantification of TSP1 pixels normalized to total VEC pixels per field from P6 (n = 3), 3 month old (n = 2), and 15 month old (n = 2) mice. One-way ANOVA followed by Bonferroni's post hoc test, ** P = 0.001; **** P < 0.0001. ( G ) Sagittal cryosection (14
Conjugate: Biotin