MA5-13398
antibody from Invitrogen Antibodies
Targeting: THBS1
THBS, THBS-1, TSP, TSP-1, TSP1
Antibody data
- Antibody Data
- Antigen structure
- References [149]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
- Flow cytometry [4]
- Other assay [25]
Submit
Validation data
Reference
Comment
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- Product number
- MA5-13398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thrombospondin 1 Monoclonal Antibody (A6.1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-13398 targets Thrombospondin in FACS, ICC/IF, IHC (P), IM, IP, and WB applications and shows reactivity with Bovine, Canine, Equine, Human, mouse, Ovine, Porcine, and Rat samples. The MA5-13398 immunogen is reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- A6.1
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Intracerebral hemorrhage alters α2δ1 and thrombospondin expression in rats.
Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion Injury.
A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes.
Extracellular Vesicles Secreted by Corneal Myofibroblasts Promote Corneal Epithelial Cell Migration.
C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells.
Super-resolution imaging of platelet-activation process and its quantitative analysis.
High Glucose Treatment Limits Drosha Protein Expression and Alters AngiomiR Maturation in Microvascular Primary Endothelial Cells via an Mdm2-dependent Mechanism.
Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration.
Oxidation of HMGB1 Is a Dynamically Regulated Process in Physiological and Pathological Conditions.
Mithramycin A and Mithralog EC-8042 Inhibit SETDB1 Expression and Its Oncogenic Activity in Malignant Melanoma.
hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells.
Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis.
Hyperglycaemia correlates with skeletal muscle capillary regression and is associated with alterations in the murine double minute-2/forkhead box O1/thrombospondin-1 pathway in type 1 diabetic BioBreeding rats.
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Neutrophil-Mediated Proteolysis of Thrombospondin-1 Promotes Platelet Adhesion and String Formation.
PEDF expression affects the oxidative and inflammatory state of choroidal endothelial cells.
A Cross Talk between Neuronal Urokinase-type Plasminogen Activator (uPA) and Astrocytic uPA Receptor (uPAR) Promotes Astrocytic Activation and Synaptic Recovery in the Ischemic Brain.
The effects of voluntary exercise and prazosin on capillary rarefaction and metabolism in streptozotocin-induced diabetic male rats.
C3G promotes a selective release of angiogenic factors from activated mouse platelets to regulate angiogenesis and tumor metastasis.
Prazosin Can Prevent Glucocorticoid Mediated Capillary Rarefaction.
Methotrexate and its therapeutic antagonists caffeine and theophylline, target a motogenic T-cell mechanism driven by thrombospondin-1 (TSP-1).
Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain.
HIF-2α-mediated induction of pulmonary thrombospondin-1 contributes to hypoxia-driven vascular remodelling and vasoconstriction.
Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes.
Dysregulation of astrocyte extracellular signaling in Costello syndrome.
Effect of LSKL peptide on thrombospondin 1-mediated transforming growth factor β signal activation and liver regeneration after hepatectomy in an experimental model.
NF-κB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation.
High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.
Expression of thrombospondin-1 modulates the angioinflammatory phenotype of choroidal endothelial cells.
Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1.
PEDF expression regulates the proangiogenic and proinflammatory phenotype of the lung endothelium.
Long-term gene therapy with thrombospondin 2 inhibits TGF-β activation, inflammation and angiogenesis in chronic allograft nephropathy.
Inflammatory cytokine-specific alterations in retinal endothelial cell function.
Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress.
ARID1a-DNA interactions are required for promoter occupancy by SWI/SNF.
Aberrant production of extracellular matrix proteins and dysfunction in kidney endothelial cells with a short duration of diabetes.
A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation.
Endogenous thrombospondin-1 regulates leukocyte recruitment and activation and accelerates death from systemic candidiasis.
The histone deacetylase inhibitor butyroyloxymethyl diethylphosphate (AN-7) protects normal cells against toxicity of anticancer agents while augmenting their anticancer activity.
Novel tissue-specific mechanism of regulation of angiogenesis and cancer growth in response to hyperglycemia.
A histone deacetylase inhibitory prodrug - butyroyloxymethyl diethyl phosphate - protects the heart and cardiomyocytes against ischemia injury.
BIM deficiency differentially impacts the function of kidney endothelial and epithelial cells through modulation of their local microenvironment.
Localization of thrombospondin-1 and myofibroblasts during corneal wound repair.
Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer.
Role of JNK/ATF-2 pathway in inhibition of thrombospondin-1 (TSP-1) expression and apoptosis mediated by doxorubicin and camptothecin in FTC-133 cells.
Differential hypermethylation of genes in vulvar cancer and lichen sclerosus coexisting or not with vulvar cancer.
Expression of ADAMTS1 in endothelial cells is induced by shear stress and suppressed in sprouting capillaries.
Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice.
The myc-miR-17~92 axis blunts TGF{beta} signaling and production of multiple TGF{beta}-dependent antiangiogenic factors.
Opposing effects of bim and bcl-2 on lung endothelial cell migration.
Type IV collagen induces expression of thrombospondin-1 that is mediated by integrin alpha1beta1 in astrocytes.
Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors.
Platelet-derived thrombospondin-1 is a critical negative regulator and potential biomarker of angiogenesis.
The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1.
PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions.
Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation.
VEGFR2 heterogeneity and response to anti-angiogenic low dose metronomic cyclophosphamide treatment.
Impaired function of circulating CD34(+) CD45(-) cells in patients with proliferative diabetic retinopathy.
Angiotensin II sustains brain inflammation in mice via TGF-beta.
Klf15 deficiency is a molecular link between heart failure and aortic aneurysm formation.
Differential expression of pancreatitis-associated protein and thrombospondins in arterial versus venous tissues.
Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells.
Estrogen regulation of thrombospondin-1 in human breast cancer cells.
Vascular endothelial growth factor inhibition by dRK6 causes endothelial apoptosis, fibrosis, and inflammation in the heart via the Akt/eNOS axis in db/db mice.
Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation.
Immune versus thrombotic stimulation of platelets differentially regulates signalling pathways, intracellular protein-protein interactions, and alpha-granule release.
Thrombospondin-1-CD47 blockade and exogenous nitrite enhance ischemic tissue survival, blood flow and angiogenesis via coupled NO-cGMP pathway activation.
Attenuation of proliferation and migration of retinal pericytes in the absence of thrombospondin-1.
Disruption of the thrombospondin-2 gene alters the lamellar morphology but does not permit vascularization of the adult mouse lumbar disc.
Hypermethylation of the thrombospondin-1 gene is associated with poor prognosis in penile squamous cell carcinoma.
Capillary sprout endothelial cells exhibit a CD36 low phenotype: regulation by shear stress and vascular endothelial growth factor-induced mechanism for attenuating anti-proliferative thrombospondin-1 signaling.
Phosphatidylserine-positive erythrocytes bind to immobilized and soluble thrombospondin-1 via its heparin-binding domain.
Post-transcriptional gene regulation by HuR promotes a more tumorigenic phenotype.
Treatment of liver ischemia-reperfusion injury by limiting thrombospondin-1/CD47 signaling.
High serum levels of thrombospondin-1 in patients with idiopathic interstitial pneumonia.
Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2.
Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets.
The aberrant methylation of TSP1 suppresses TGF-beta1 activation in colorectal cancer.
Meteorin regulates angiogenesis at the gliovascular interface.
Cell type-specific post-transcriptional regulation of production of the potent antiangiogenic and proatherogenic protein thrombospondin-1 by high glucose.
High glucose promotes retinal endothelial cell migration through activation of Src, PI3K/Akt1/eNOS, and ERKs.
Androgenic dependence of exophytic tumor growth in a transgenic mouse model of bladder cancer: a role for thrombospondin-1.
Constitutive upregulation of the transforming growth factor-beta pathway in rheumatoid arthritis synovial fibroblasts.
Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1.
Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1.
Immunochemical analysis of the structure of the signature domains of thrombospondin-1 and thrombospondin-2 in low calcium concentrations.
Differential regulation of thrombospondin-1 expression and antiangiogenesis of ECV304 cells by trichostatin A and helixor A.
Thrombospondin-1 expression and localization in the developing ovine lung.
Bcl-2 expression modulates cell adhesion and migration promoting branching of ureteric bud cells.
Increasing survival of ischemic tissue by targeting CD47.
Thrombospondin-1 limits ischemic tissue survival by inhibiting nitric oxide-mediated vascular smooth muscle relaxation.
PECAM-1 isoform-specific regulation of kidney endothelial cell migration and capillary morphogenesis.
Phase 2 trial of oral vorinostat (suberoylanilide hydroxamic acid, SAHA) for refractory cutaneous T-cell lymphoma (CTCL).
PPARalpha deficiency in inflammatory cells suppresses tumor growth.
Glycosylation mediates up-regulation of a potent antiangiogenic and proatherogenic protein, thrombospondin-1, by glucose in vascular smooth muscle cells.
The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats.
Isolation and characterization of corneal endothelial cells from wild type and thrombospondin-1 deficient mice.
Expression of thrombospondin 1 (TSP 1) in patients with uterine smooth muscle tumors: an immunohistochemical study.
Sustained regression of tumors upon MYC inactivation requires p53 or thrombospondin-1 to reverse the angiogenic switch.
HER2 signaling modulates the equilibrium between pro- and antiangiogenic factors via distinct pathways: implications for HER2-targeted antibody therapy.
Versican-thrombospondin-1 binding in vitro and colocalization in microfibrils induced by inflammation on vascular smooth muscle cells.
Enhanced proangiogenic signaling in thrombospondin-1-deficient retinal endothelial cells.
Islet endothelial cells and pancreatic beta-cell proliferation: studies in vitro and during pregnancy in adult rats.
Thrombospondins deployed by thrombopoietic cells determine angiogenic switch and extent of revascularization.
Receptor for advanced glycation end products is involved in impaired angiogenic response in diabetes.
Thrombospondins, metallo proteases and thrombospondin receptors messenger RNA and protein expression in different tumour sublines of the Dunning prostate cancer model.
Expression of thrombospondin-1 in resected colorectal liver metastases predicts poor prognosis.
1,25-dihydroxyvitamin D inhibits renal interstitial myofibroblast activation by inducing hepatocyte growth factor expression.
Different involvement of extracellular matrix components in small and large arteries during chronic NO synthase inhibition.
Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling.
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction.
Wnt signaling can repress thrombospondin-1 expression in colonic tumorigenesis.
Kinetics of biglycan, decorin and thrombospondin-1 in mercuric chloride-induced renal tubulointerstitial fibrosis.
Isolation and characterization of murine retinal astrocytes.
Endogenous thrombospondin-1 is not necessary for proliferation but is permissive for vascular smooth muscle cell responses to platelet-derived growth factor.
Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice.
Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice.
Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats.
Role of thrombospondin-1 in the autologous phase of an accelerated model of anti-glomerular basement membrane glomerulonephritis.
Thrombospondin-1 in the trabecular meshwork: localization in normal and glaucomatous eyes, and induction by TGF-beta1 and dexamethasone in vitro.
Thombospondin-1 disrupts estrogen-induced endothelial cell proliferation and migration and its expression is suppressed by estradiol.
Thrombospondin-1 accelerates wound healing of corneal epithelia.
Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct cells from bcl-2 -/- mice.
Significance of CD 105 expression for tumour angiogenesis and prognosis in endometrial carcinomas.
Significance of CD 105 expression for tumour angiogenesis and prognosis in endometrial carcinomas.
Isolation and characterization of murine retinal endothelial cells.
Ras modulates Myc activity to repress thrombospondin-1 expression and increase tumor angiogenesis.
Connexin26 regulates the expression of angiogenesis-related genes in human breast tumor cells by both GJIC-dependent and -independent mechanisms.
Connexin26 regulates the expression of angiogenesis-related genes in human breast tumor cells by both GJIC-dependent and -independent mechanisms.
Regulation of multiple tumor microenvironment markers by overexpression of single or paired combinations of ErbB receptors.
Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat.
Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells.
Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells.
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
Characteristics of long-term survivors of untreated lung cancer.
Characteristics of long-term survivors of untreated lung cancer.
Investigation of the role of nitric oxide synthase 2 in pregnancy using mutant mice.
Investigation of the role of nitric oxide synthase 2 in pregnancy using mutant mice.
In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin.
Overexpression of thrombospondin-1 reduces growth and vascular index but not perfusion in glioblastoma.
Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast.
Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast.
T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components.
Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts.
Association of thrombospondin-1 and cardiac allograft vasculopathy in human cardiac allografts.
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Wang B, Li X, Yu N, Yang L, Nan C, Guo L, Zhao Z
Experimental and therapeutic medicine 2022 May;23(5):327
Experimental and therapeutic medicine 2022 May;23(5):327
Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion Injury.
Shiau AL, Fang SY, Hsu CH, Chiu MH, Lam CF, Wu CL, Roan JN
Acta Cardiologica Sinica 2022 Mar;38(2):187-200
Acta Cardiologica Sinica 2022 Mar;38(2):187-200
A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes.
Wallis S, Wolska N, Englert H, Posner M, Upadhyay A, Renné T, Eggleston I, Bagby S, Pula G
Journal of thrombosis and haemostasis : JTH 2022 Mar;20(3):729-741
Journal of thrombosis and haemostasis : JTH 2022 Mar;20(3):729-741
Extracellular Vesicles Secreted by Corneal Myofibroblasts Promote Corneal Epithelial Cell Migration.
Yeung V, Zhang TC, Yuan L, Parekh M, Cortinas JA, Delavogia E, Hutcheon AEK, Guo X, Ciolino JB
International journal of molecular sciences 2022 Mar 15;23(6)
International journal of molecular sciences 2022 Mar 15;23(6)
C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells.
Sterken BA, Ackermann T, Müller C, Zuidhof HR, Kortman G, Hernandez-Segura A, Broekhuis M, Spierings D, Guryev V, Calkhoven CF
NPJ breast cancer 2022 Jan 18;8(1):11
NPJ breast cancer 2022 Jan 18;8(1):11
Super-resolution imaging of platelet-activation process and its quantitative analysis.
Chung J, Jeong D, Kim GH, Go S, Song J, Moon E, Huh YH, Kim D
Scientific reports 2021 May 18;11(1):10511
Scientific reports 2021 May 18;11(1):10511
High Glucose Treatment Limits Drosha Protein Expression and Alters AngiomiR Maturation in Microvascular Primary Endothelial Cells via an Mdm2-dependent Mechanism.
Lam B, Nwadozi E, Haas TL, Birot O, Roudier E
Cells 2021 Mar 27;10(4)
Cells 2021 Mar 27;10(4)
Meteorin Is a Novel Therapeutic Target for Wet Age-Related Macular Degeneration.
Delaunay K, Sellam A, Dinet V, Moulin A, Zhao M, Gelizé E, Canonica J, Naud MC, Crisanti-Lassiaz P, Behar-Cohen F
Journal of clinical medicine 2021 Jul 2;10(13)
Journal of clinical medicine 2021 Jul 2;10(13)
Oxidation of HMGB1 Is a Dynamically Regulated Process in Physiological and Pathological Conditions.
Ferrara M, Chialli G, Ferreira LM, Ruggieri E, Careccia G, Preti A, Piccirillo R, Bianchi ME, Sitia G, Venereau E
Frontiers in immunology 2020;11:1122
Frontiers in immunology 2020;11:1122
Mithramycin A and Mithralog EC-8042 Inhibit SETDB1 Expression and Its Oncogenic Activity in Malignant Melanoma.
Federico A, Steinfass T, Larribère L, Novak D, Morís F, Núñez LE, Umansky V, Utikal J
Molecular therapy oncolytics 2020 Sep 25;18:83-99
Molecular therapy oncolytics 2020 Sep 25;18:83-99
hnRNPA2B1 inhibits the exosomal export of miR-503 in endothelial cells.
Pérez-Boza J, Boeckx A, Lion M, Dequiedt F, Struman I
Cellular and molecular life sciences : CMLS 2020 Nov;77(21):4413-4428
Cellular and molecular life sciences : CMLS 2020 Nov;77(21):4413-4428
Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis.
Esteban S, Clemente C, Koziol A, Gonzalo P, Rius C, Martínez F, Linares PM, Chaparro M, Urzainqui A, Andrés V, Seiki M, Gisbert JP, Arroyo AG
EMBO molecular medicine 2020 Feb 7;12(2):e10862
EMBO molecular medicine 2020 Feb 7;12(2):e10862
Hyperglycaemia correlates with skeletal muscle capillary regression and is associated with alterations in the murine double minute-2/forkhead box O1/thrombospondin-1 pathway in type 1 diabetic BioBreeding rats.
Aiken J, Mandel ER, Riddell MC, Birot O
Diabetes & vascular disease research 2019 Jan;16(1):28-37
Diabetes & vascular disease research 2019 Jan;16(1):28-37
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Waddingham MT, Sonobe T, Tsuchimochi H, Edgley AJ, Sukumaran V, Chen YC, Hansra SS, Schwenke DO, Umetani K, Aoyama K, Yagi N, Kelly DJ, Gaderi S, Herwig M, Kolijn D, Mügge A, Paulus WJ, Ogo T, Shirai M, Hamdani N, Pearson JT
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
Neutrophil-Mediated Proteolysis of Thrombospondin-1 Promotes Platelet Adhesion and String Formation.
Seif K, Alidzanovic L, Tischler B, Ibrahim N, Zagrapan B, Rauscher S, Salzmann M, Hell L, Mauracher LM, Budde U, Schmid JA, Jilma B, Pabinger I, Assinger A, Starlinger P, Brostjan C
Thrombosis and haemostasis 2018 Dec;118(12):2074-2085
Thrombosis and haemostasis 2018 Dec;118(12):2074-2085
PEDF expression affects the oxidative and inflammatory state of choroidal endothelial cells.
Farnoodian M, Sorenson CM, Sheibani N
American journal of physiology. Cell physiology 2018 Apr 1;314(4):C456-C472
American journal of physiology. Cell physiology 2018 Apr 1;314(4):C456-C472
A Cross Talk between Neuronal Urokinase-type Plasminogen Activator (uPA) and Astrocytic uPA Receptor (uPAR) Promotes Astrocytic Activation and Synaptic Recovery in the Ischemic Brain.
Diaz A, Merino P, Manrique LG, Ospina JP, Cheng L, Wu F, Jeanneret V, Yepes M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Oct 25;37(43):10310-10322
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Oct 25;37(43):10310-10322
The effects of voluntary exercise and prazosin on capillary rarefaction and metabolism in streptozotocin-induced diabetic male rats.
Dunford EC, Leclair E, Aiken J, Mandel ER, Haas TL, Birot O, Riddell MC
Journal of applied physiology (Bethesda, Md. : 1985) 2017 Mar 1;122(3):492-502
Journal of applied physiology (Bethesda, Md. : 1985) 2017 Mar 1;122(3):492-502
C3G promotes a selective release of angiogenic factors from activated mouse platelets to regulate angiogenesis and tumor metastasis.
Martín-Granado V, Ortiz-Rivero S, Carmona R, Gutiérrez-Herrero S, Barrera M, San-Segundo L, Sequera C, Perdiguero P, Lozano F, Martín-Herrero F, González-Porras JR, Muñoz-Chápuli R, Porras A, Guerrero C
Oncotarget 2017 Dec 19;8(67):110994-111011
Oncotarget 2017 Dec 19;8(67):110994-111011
Prazosin Can Prevent Glucocorticoid Mediated Capillary Rarefaction.
Mandel ER, Dunford EC, Trifonova A, Abdifarkosh G, Teich T, Riddell MC, Haas TL
PloS one 2016;11(11):e0166899
PloS one 2016;11(11):e0166899
Methotrexate and its therapeutic antagonists caffeine and theophylline, target a motogenic T-cell mechanism driven by thrombospondin-1 (TSP-1).
Talme T, Bergdahl E, Sundqvist KG
European journal of immunology 2016 May;46(5):1279-90
European journal of immunology 2016 May;46(5):1279-90
Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain.
Kim SK, Hayashi H, Ishikawa T, Shibata K, Shigetomi E, Shinozaki Y, Inada H, Roh SE, Kim SJ, Lee G, Bae H, Moorhouse AJ, Mikoshiba K, Fukazawa Y, Koizumi S, Nabekura J
The Journal of clinical investigation 2016 May 2;126(5):1983-97
The Journal of clinical investigation 2016 May 2;126(5):1983-97
HIF-2α-mediated induction of pulmonary thrombospondin-1 contributes to hypoxia-driven vascular remodelling and vasoconstriction.
Labrousse-Arias D, Castillo-González R, Rogers NM, Torres-Capelli M, Barreira B, Aragonés J, Cogolludo Á, Isenberg JS, Calzada MJ
Cardiovascular research 2016 Jan 1;109(1):115-30
Cardiovascular research 2016 Jan 1;109(1):115-30
Extracellular Fibrinogen-binding Protein (Efb) from Staphylococcus aureus Inhibits the Formation of Platelet-Leukocyte Complexes.
Posner MG, Upadhyay A, Abubaker AA, Fortunato TM, Vara D, Canobbio I, Bagby S, Pula G
The Journal of biological chemistry 2016 Feb 5;291(6):2764-76
The Journal of biological chemistry 2016 Feb 5;291(6):2764-76
Dysregulation of astrocyte extracellular signaling in Costello syndrome.
Krencik R, Hokanson KC, Narayan AR, Dvornik J, Rooney GE, Rauen KA, Weiss LA, Rowitch DH, Ullian EM
Science translational medicine 2015 May 6;7(286):286ra66
Science translational medicine 2015 May 6;7(286):286ra66
Effect of LSKL peptide on thrombospondin 1-mediated transforming growth factor β signal activation and liver regeneration after hepatectomy in an experimental model.
Kuroki H, Hayashi H, Nakagawa S, Sakamoto K, Higashi T, Nitta H, Hashimoto D, Chikamoto A, Beppu T, Baba H
The British journal of surgery 2015 Jun;102(7):813-25
The British journal of surgery 2015 Jun;102(7):813-25
NF-κB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation.
de la Rica L, García-Gómez A, Comet NR, Rodríguez-Ubreva J, Ciudad L, Vento-Tormo R, Company C, Álvarez-Errico D, García M, Gómez-Vaquero C, Ballestar E
Genome biology 2015 Jan 5;16(1):2
Genome biology 2015 Jan 5;16(1):2
High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.
Shin ES, Huang Q, Gurel Z, Sorenson CM, Sheibani N
PloS one 2014;9(7):e103148
PloS one 2014;9(7):e103148
Expression of thrombospondin-1 modulates the angioinflammatory phenotype of choroidal endothelial cells.
Fei P, Zaitoun I, Farnoodian M, Fisk DL, Wang S, Sorenson CM, Sheibani N
PloS one 2014;9(12):e116423
PloS one 2014;9(12):e116423
Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1.
Talme T, Bergdahl E, Sundqvist KG
Immunology 2014 Jun;142(2):176-92
Immunology 2014 Jun;142(2):176-92
PEDF expression regulates the proangiogenic and proinflammatory phenotype of the lung endothelium.
Shin ES, Sorenson CM, Sheibani N
American journal of physiology. Lung cellular and molecular physiology 2014 Apr 1;306(7):L620-34
American journal of physiology. Lung cellular and molecular physiology 2014 Apr 1;306(7):L620-34
Long-term gene therapy with thrombospondin 2 inhibits TGF-β activation, inflammation and angiogenesis in chronic allograft nephropathy.
Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
PloS one 2013;8(12):e83846
PloS one 2013;8(12):e83846
Inflammatory cytokine-specific alterations in retinal endothelial cell function.
Palenski TL, Sorenson CM, Sheibani N
Microvascular research 2013 Sep;89:57-69
Microvascular research 2013 Sep;89:57-69
Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress.
Zhao Y, Wang S, Sorenson CM, Teixeira L, Dubielzig RR, Peters DM, Conway SJ, Jefcoate CR, Sheibani N
Molecular and cellular biology 2013 Nov;33(21):4225-40
Molecular and cellular biology 2013 Nov;33(21):4225-40
ARID1a-DNA interactions are required for promoter occupancy by SWI/SNF.
Chandler RL, Brennan J, Schisler JC, Serber D, Patterson C, Magnuson T
Molecular and cellular biology 2013 Jan;33(2):265-80
Molecular and cellular biology 2013 Jan;33(2):265-80
Aberrant production of extracellular matrix proteins and dysfunction in kidney endothelial cells with a short duration of diabetes.
Grutzmacher C, Park S, Zhao Y, Morrison ME, Sheibani N, Sorenson CM
American journal of physiology. Renal physiology 2013 Jan 1;304(1):F19-30
American journal of physiology. Renal physiology 2013 Jan 1;304(1):F19-30
A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation.
Bergström SE, Bergdahl E, Sundqvist KG
Immunology 2013 Dec;140(4):441-55
Immunology 2013 Dec;140(4):441-55
Endogenous thrombospondin-1 regulates leukocyte recruitment and activation and accelerates death from systemic candidiasis.
Martin-Manso G, Navarathna DH, Galli S, Soto-Pantoja DR, Kuznetsova SA, Tsokos M, Roberts DD
PloS one 2012;7(11):e48775
PloS one 2012;7(11):e48775
The histone deacetylase inhibitor butyroyloxymethyl diethylphosphate (AN-7) protects normal cells against toxicity of anticancer agents while augmenting their anticancer activity.
Tarasenko N, Kessler-Icekson G, Boer P, Inbal A, Schlesinger H, Phillips DR, Cutts SM, Nudelman A, Rephaeli A
Investigational new drugs 2012 Feb;30(1):130-43
Investigational new drugs 2012 Feb;30(1):130-43
Novel tissue-specific mechanism of regulation of angiogenesis and cancer growth in response to hyperglycemia.
Bhattacharyya S, Sul K, Krukovets I, Nestor C, Li J, Adognravi OS
Journal of the American Heart Association 2012 Dec;1(6):e005967
Journal of the American Heart Association 2012 Dec;1(6):e005967
A histone deacetylase inhibitory prodrug - butyroyloxymethyl diethyl phosphate - protects the heart and cardiomyocytes against ischemia injury.
Kessler-Icekson G, Hochhauser E, Sinai T, Kremer A, Dick J, Tarasenko N, Nudelman V, Schlesinger H, Abraham S, Nudelman A, Rephaeli A
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2012 Apr 11;45(5):592-9
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2012 Apr 11;45(5):592-9
BIM deficiency differentially impacts the function of kidney endothelial and epithelial cells through modulation of their local microenvironment.
Sheibani N, Morrison ME, Gurel Z, Park S, Sorenson CM
American journal of physiology. Renal physiology 2012 Apr 1;302(7):F809-19
American journal of physiology. Renal physiology 2012 Apr 1;302(7):F809-19
Localization of thrombospondin-1 and myofibroblasts during corneal wound repair.
Matsuba M, Hutcheon AE, Zieske JD
Experimental eye research 2011 Oct;93(4):534-40
Experimental eye research 2011 Oct;93(4):534-40
Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer.
Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, Paun BC, Jin Z, Olaru AV, Hamilton JP, Selaru FM, Yang J, Matsumura N, Shimizu K, Abraham JM, Shimada Y, Mori Y, Meltzer SJ
International journal of cancer 2011 Nov 1;129(9):2134-46
International journal of cancer 2011 Nov 1;129(9):2134-46
Role of JNK/ATF-2 pathway in inhibition of thrombospondin-1 (TSP-1) expression and apoptosis mediated by doxorubicin and camptothecin in FTC-133 cells.
El Btaouri H, Morjani H, Greffe Y, Charpentier E, Martiny L
Biochimica et biophysica acta 2011 May;1813(5):695-703
Biochimica et biophysica acta 2011 May;1813(5):695-703
Differential hypermethylation of genes in vulvar cancer and lichen sclerosus coexisting or not with vulvar cancer.
Guerrero D, Guarch R, Ojer A, Casas JM, Méndez-Meca C, Esteller M, Barba-Ramos E, Garcia-Bragado F, Puras A
International journal of cancer 2011 Jun 15;128(12):2853-64
International journal of cancer 2011 Jun 15;128(12):2853-64
Expression of ADAMTS1 in endothelial cells is induced by shear stress and suppressed in sprouting capillaries.
Hohberg M, Knöchel J, Hoffmann CJ, Chlench S, Wunderlich W, Alter A, Maroski J, Vorderwülbecke BJ, Da Silva-Azevedo L, Knudsen R, Lehmann R, Fiedorowicz K, Bongrazio M, Nitsche B, Hoepfner M, Styp-Rekowska B, Pries AR, Zakrzewicz A
Journal of cellular physiology 2011 Feb;226(2):350-61
Journal of cellular physiology 2011 Feb;226(2):350-61
Effect of Qingre Quyu Granule (清热祛瘀颗粒) on stabilizing plaques in the brachiocephalic artery of apolipoprotein E deficient mice.
Wang Y, Cheng WL, Ke YN, Cai Z, Chen L, Xi Y, Wang P, Guo J, Li H, Huang CX
Chinese journal of integrative medicine 2010 Oct;16(5):442-7
Chinese journal of integrative medicine 2010 Oct;16(5):442-7
The myc-miR-17~92 axis blunts TGF{beta} signaling and production of multiple TGF{beta}-dependent antiangiogenic factors.
Dews M, Fox JL, Hultine S, Sundaram P, Wang W, Liu YY, Furth E, Enders GH, El-Deiry W, Schelter JM, Cleary MA, Thomas-Tikhonenko A
Cancer research 2010 Oct 15;70(20):8233-46
Cancer research 2010 Oct 15;70(20):8233-46
Opposing effects of bim and bcl-2 on lung endothelial cell migration.
Grutzmacher C, Park S, Elmergreen TL, Tang Y, Scheef EA, Sheibani N, Sorenson CM
American journal of physiology. Lung cellular and molecular physiology 2010 Nov;299(5):L607-20
American journal of physiology. Lung cellular and molecular physiology 2010 Nov;299(5):L607-20
Type IV collagen induces expression of thrombospondin-1 that is mediated by integrin alpha1beta1 in astrocytes.
Yonezawa T, Hattori S, Inagaki J, Kurosaki M, Takigawa T, Hirohata S, Miyoshi T, Ninomiya Y
Glia 2010 May;58(7):755-67
Glia 2010 May;58(7):755-67
Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors.
Olsson A, Björk A, Vallon-Christersson J, Isaacs JT, Leanderson T
Molecular cancer 2010 May 17;9:107
Molecular cancer 2010 May 17;9:107
Platelet-derived thrombospondin-1 is a critical negative regulator and potential biomarker of angiogenesis.
Zaslavsky A, Baek KH, Lynch RC, Short S, Grillo J, Folkman J, Italiano JE Jr, Ryeom S
Blood 2010 Jun 3;115(22):4605-13
Blood 2010 Jun 3;115(22):4605-13
The ubiquitin ligase Nedd4-1 is required for heart development and is a suppressor of thrombospondin-1.
Fouladkou F, Lu C, Jiang C, Zhou L, She Y, Walls JR, Kawabe H, Brose N, Henkelman RM, Huang A, Bruneau BG, Rotin D
The Journal of biological chemistry 2010 Feb 26;285(9):6770-80
The Journal of biological chemistry 2010 Feb 26;285(9):6770-80
PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions.
Park S, DiMaio TA, Scheef EA, Sorenson CM, Sheibani N
American journal of physiology. Cell physiology 2010 Dec;299(6):C1468-84
American journal of physiology. Cell physiology 2010 Dec;299(6):C1468-84
Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation.
Ikeda H, Miyatake M, Koshikawa N, Ochiai K, Yamada K, Kiss A, Donlin MJ, Panneton WM, Churchill JD, Green M, Siddiqui AM, Leinweber AL, Crews NR, Ezerskiy LA, Rendell VR, Belcheva MM, Coscia CJ
The Journal of biological chemistry 2010 Dec 3;285(49):38415-27
The Journal of biological chemistry 2010 Dec 3;285(49):38415-27
VEGFR2 heterogeneity and response to anti-angiogenic low dose metronomic cyclophosphamide treatment.
Patten SG, Adamcic U, Lacombe K, Minhas K, Skowronski K, Coomber BL
BMC cancer 2010 Dec 15;10:683
BMC cancer 2010 Dec 15;10:683
Impaired function of circulating CD34(+) CD45(-) cells in patients with proliferative diabetic retinopathy.
Tan K, Lessieur E, Cutler A, Nerone P, Vasanji A, Asosingh K, Erzurum S, Anand-Apte B
Experimental eye research 2010 Aug;91(2):229-37
Experimental eye research 2010 Aug;91(2):229-37
Angiotensin II sustains brain inflammation in mice via TGF-beta.
Lanz TV, Ding Z, Ho PP, Luo J, Agrawal AN, Srinagesh H, Axtell R, Zhang H, Platten M, Wyss-Coray T, Steinman L
The Journal of clinical investigation 2010 Aug;120(8):2782-94
The Journal of clinical investigation 2010 Aug;120(8):2782-94
Klf15 deficiency is a molecular link between heart failure and aortic aneurysm formation.
Haldar SM, Lu Y, Jeyaraj D, Kawanami D, Cui Y, Eapen SJ, Hao C, Li Y, Doughman YQ, Watanabe M, Shimizu K, Kuivaniemi H, Sadoshima J, Margulies KB, Cappola TP, Jain MK
Science translational medicine 2010 Apr 7;2(26):26ra26
Science translational medicine 2010 Apr 7;2(26):26ra26
Differential expression of pancreatitis-associated protein and thrombospondins in arterial versus venous tissues.
Szasz T, Eddy S, Paulauskis J, Burnett R, Ellekilde M, Iovanna JL, Watts SW
Journal of vascular research 2009;46(6):551-60
Journal of vascular research 2009;46(6):551-60
Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells.
Hyder SM, Liang Y, Wu J, Welbern V
Endocrine-related cancer 2009 Sep;16(3):809-17
Endocrine-related cancer 2009 Sep;16(3):809-17
Estrogen regulation of thrombospondin-1 in human breast cancer cells.
Hyder SM, Liang Y, Wu J
International journal of cancer 2009 Sep 1;125(5):1045-53
International journal of cancer 2009 Sep 1;125(5):1045-53
Vascular endothelial growth factor inhibition by dRK6 causes endothelial apoptosis, fibrosis, and inflammation in the heart via the Akt/eNOS axis in db/db mice.
Park CW, Kim HW, Lim JH, Yoo KD, Chung S, Shin SJ, Chung HW, Lee SJ, Chae CB, Kim YS, Chang YS
Diabetes 2009 Nov;58(11):2666-76
Diabetes 2009 Nov;58(11):2666-76
Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation.
Daniel C, Wagner A, Hohenstein B, Hugo C
American journal of physiology. Renal physiology 2009 Nov;297(5):F1299-309
American journal of physiology. Renal physiology 2009 Nov;297(5):F1299-309
Immune versus thrombotic stimulation of platelets differentially regulates signalling pathways, intracellular protein-protein interactions, and alpha-granule release.
Rex S, Beaulieu LM, Perlman DH, Vitseva O, Blair PS, McComb ME, Costello CE, Freedman JE
Thrombosis and haemostasis 2009 Jul;102(1):97-110
Thrombosis and haemostasis 2009 Jul;102(1):97-110
Thrombospondin-1-CD47 blockade and exogenous nitrite enhance ischemic tissue survival, blood flow and angiogenesis via coupled NO-cGMP pathway activation.
Isenberg JS, Shiva S, Gladwin M
Nitric oxide : biology and chemistry 2009 Aug;21(1):52-62
Nitric oxide : biology and chemistry 2009 Aug;21(1):52-62
Attenuation of proliferation and migration of retinal pericytes in the absence of thrombospondin-1.
Scheef EA, Sorenson CM, Sheibani N
American journal of physiology. Cell physiology 2009 Apr;296(4):C724-34
American journal of physiology. Cell physiology 2009 Apr;296(4):C724-34
Disruption of the thrombospondin-2 gene alters the lamellar morphology but does not permit vascularization of the adult mouse lumbar disc.
Gruber HE, Bornstein P, Sage EH, Ingram JA, Zinchenko N, Norton HJ, Hanley EN Jr
Arthritis research & therapy 2008;10(4):R96
Arthritis research & therapy 2008;10(4):R96
Hypermethylation of the thrombospondin-1 gene is associated with poor prognosis in penile squamous cell carcinoma.
Guerrero D, Guarch R, Ojer A, Casas JM, Ropero S, Mancha A, Pesce C, Lloveras B, Garcia-Bragado F, Puras A
BJU international 2008 Sep;102(6):747-55
BJU international 2008 Sep;102(6):747-55
Capillary sprout endothelial cells exhibit a CD36 low phenotype: regulation by shear stress and vascular endothelial growth factor-induced mechanism for attenuating anti-proliferative thrombospondin-1 signaling.
Anderson CR, Hastings NE, Blackman BR, Price RJ
The American journal of pathology 2008 Oct;173(4):1220-8
The American journal of pathology 2008 Oct;173(4):1220-8
Phosphatidylserine-positive erythrocytes bind to immobilized and soluble thrombospondin-1 via its heparin-binding domain.
Gayen Betal S, Setty BN
Translational research : the journal of laboratory and clinical medicine 2008 Oct;152(4):165-77
Translational research : the journal of laboratory and clinical medicine 2008 Oct;152(4):165-77
Post-transcriptional gene regulation by HuR promotes a more tumorigenic phenotype.
Mazan-Mamczarz K, Hagner PR, Corl S, Srikantan S, Wood WH, Becker KG, Gorospe M, Keene JD, Levenson AS, Gartenhaus RB
Oncogene 2008 Oct 16;27(47):6151-63
Oncogene 2008 Oct 16;27(47):6151-63
Treatment of liver ischemia-reperfusion injury by limiting thrombospondin-1/CD47 signaling.
Isenberg JS, Maxhimer JB, Powers P, Tsokos M, Frazier WA, Roberts DD
Surgery 2008 Nov;144(5):752-61
Surgery 2008 Nov;144(5):752-61
High serum levels of thrombospondin-1 in patients with idiopathic interstitial pneumonia.
Ide M, Ishii H, Mukae H, Iwata A, Sakamoto N, Kadota J, Kohno S
Respiratory medicine 2008 Nov;102(11):1625-30
Respiratory medicine 2008 Nov;102(11):1625-30
Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2.
Kondo S, Tang Y, Scheef EA, Sheibani N, Sorenson CM
American journal of physiology. Cell physiology 2008 Jun;294(6):C1521-30
American journal of physiology. Cell physiology 2008 Jun;294(6):C1521-30
Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets.
Olerud J, Johansson M, Lawler J, Welsh N, Carlsson PO
Diabetes 2008 Jul;57(7):1870-7
Diabetes 2008 Jul;57(7):1870-7
The aberrant methylation of TSP1 suppresses TGF-beta1 activation in colorectal cancer.
Rojas A, Meherem S, Kim YH, Washington MK, Willis JE, Markowitz SD, Grady WM
International journal of cancer 2008 Jul 1;123(1):14-21
International journal of cancer 2008 Jul 1;123(1):14-21
Meteorin regulates angiogenesis at the gliovascular interface.
Park JA, Lee HS, Ko KJ, Park SY, Kim JH, Choe G, Kweon HS, Song HS, Ahn JC, Yu YS, Kim KW
Glia 2008 Feb;56(3):247-58
Glia 2008 Feb;56(3):247-58
Cell type-specific post-transcriptional regulation of production of the potent antiangiogenic and proatherogenic protein thrombospondin-1 by high glucose.
Bhattacharyya S, Marinic TE, Krukovets I, Hoppe G, Stenina OI
The Journal of biological chemistry 2008 Feb 29;283(9):5699-707
The Journal of biological chemistry 2008 Feb 29;283(9):5699-707
High glucose promotes retinal endothelial cell migration through activation of Src, PI3K/Akt1/eNOS, and ERKs.
Huang Q, Sheibani N
American journal of physiology. Cell physiology 2008 Dec;295(6):C1647-57
American journal of physiology. Cell physiology 2008 Dec;295(6):C1647-57
Androgenic dependence of exophytic tumor growth in a transgenic mouse model of bladder cancer: a role for thrombospondin-1.
Johnson AM, O'Connell MJ, Miyamoto H, Huang J, Yao JL, Messing EM, Reeder JE
BMC urology 2008 Apr 23;8:7
BMC urology 2008 Apr 23;8:7
Constitutive upregulation of the transforming growth factor-beta pathway in rheumatoid arthritis synovial fibroblasts.
Pohlers D, Beyer A, Koczan D, Wilhelm T, Thiesen HJ, Kinne RW
Arthritis research & therapy 2007;9(3):R59
Arthritis research & therapy 2007;9(3):R59
Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1.
Derks RA, Jankowska-Gan E, Xu Q, Burlingham WJ
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3443-51
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3443-51
Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1.
Derks RA, Jankowska-Gan E, Xu Q, Burlingham WJ
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3443-51
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3443-51
Immunochemical analysis of the structure of the signature domains of thrombospondin-1 and thrombospondin-2 in low calcium concentrations.
Annis DS, Gunderson KA, Mosher DF
The Journal of biological chemistry 2007 Sep 14;282(37):27067-27075
The Journal of biological chemistry 2007 Sep 14;282(37):27067-27075
Differential regulation of thrombospondin-1 expression and antiangiogenesis of ECV304 cells by trichostatin A and helixor A.
Hong S, Chang SY, Yeom DH, Kang JH, Hong KJ
Anti-cancer drugs 2007 Oct;18(9):1005-14
Anti-cancer drugs 2007 Oct;18(9):1005-14
Thrombospondin-1 expression and localization in the developing ovine lung.
Sozo F, Hooper SB, Wallace MJ
The Journal of physiology 2007 Oct 15;584(Pt 2):625-35
The Journal of physiology 2007 Oct 15;584(Pt 2):625-35
Bcl-2 expression modulates cell adhesion and migration promoting branching of ureteric bud cells.
Sheibani N, Scheef EA, Dimaio TA, Wang Y, Kondo S, Sorenson CM
Journal of cellular physiology 2007 Mar;210(3):616-25
Journal of cellular physiology 2007 Mar;210(3):616-25
Increasing survival of ischemic tissue by targeting CD47.
Isenberg JS, Romeo MJ, Abu-Asab M, Tsokos M, Oldenborg A, Pappan L, Wink DA, Frazier WA, Roberts DD
Circulation research 2007 Mar 16;100(5):712-20
Circulation research 2007 Mar 16;100(5):712-20
Thrombospondin-1 limits ischemic tissue survival by inhibiting nitric oxide-mediated vascular smooth muscle relaxation.
Isenberg JS, Hyodo F, Matsumoto K, Romeo MJ, Abu-Asab M, Tsokos M, Kuppusamy P, Wink DA, Krishna MC, Roberts DD
Blood 2007 Mar 1;109(5):1945-52
Blood 2007 Mar 1;109(5):1945-52
PECAM-1 isoform-specific regulation of kidney endothelial cell migration and capillary morphogenesis.
Kondo S, Scheef EA, Sheibani N, Sorenson CM
American journal of physiology. Cell physiology 2007 Jun;292(6):C2070-83
American journal of physiology. Cell physiology 2007 Jun;292(6):C2070-83
Phase 2 trial of oral vorinostat (suberoylanilide hydroxamic acid, SAHA) for refractory cutaneous T-cell lymphoma (CTCL).
Duvic M, Talpur R, Ni X, Zhang C, Hazarika P, Kelly C, Chiao JH, Reilly JF, Ricker JL, Richon VM, Frankel SR
Blood 2007 Jan 1;109(1):31-9
Blood 2007 Jan 1;109(1):31-9
PPARalpha deficiency in inflammatory cells suppresses tumor growth.
Kaipainen A, Kieran MW, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, Mulligan R, Folkman J, Panigrahy D
PloS one 2007 Feb 28;2(2):e260
PloS one 2007 Feb 28;2(2):e260
Glycosylation mediates up-regulation of a potent antiangiogenic and proatherogenic protein, thrombospondin-1, by glucose in vascular smooth muscle cells.
Raman P, Krukovets I, Marinic TE, Bornstein P, Stenina OI
The Journal of biological chemistry 2007 Feb 23;282(8):5704-14
The Journal of biological chemistry 2007 Feb 23;282(8):5704-14
The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats.
de Nigris F, Balestrieri ML, Williams-Ignarro S, D'Armiento FP, Fiorito C, Ignarro LJ, Napoli C
Nitric oxide : biology and chemistry 2007 Aug;17(1):50-4
Nitric oxide : biology and chemistry 2007 Aug;17(1):50-4
Isolation and characterization of corneal endothelial cells from wild type and thrombospondin-1 deficient mice.
Scheef EA, Huang Q, Wang S, Sorenson CM, Sheibani N
Molecular vision 2007 Aug 27;13:1483-95
Molecular vision 2007 Aug 27;13:1483-95
Expression of thrombospondin 1 (TSP 1) in patients with uterine smooth muscle tumors: an immunohistochemical study.
Bodner-Adler B, Nather A, Bodner K, Czerwenka K, Kimberger O, Leodolter S, Mayerhofer K
Gynecologic oncology 2006 Oct;103(1):186-9
Gynecologic oncology 2006 Oct;103(1):186-9
Sustained regression of tumors upon MYC inactivation requires p53 or thrombospondin-1 to reverse the angiogenic switch.
Giuriato S, Ryeom S, Fan AC, Bachireddy P, Lynch RC, Rioth MJ, van Riggelen J, Kopelman AM, Passegué E, Tang F, Folkman J, Felsher DW
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 31;103(44):16266-71
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 31;103(44):16266-71
HER2 signaling modulates the equilibrium between pro- and antiangiogenic factors via distinct pathways: implications for HER2-targeted antibody therapy.
Wen XF, Yang G, Mao W, Thornton A, Liu J, Bast RC Jr, Le XF
Oncogene 2006 Nov 2;25(52):6986-96
Oncogene 2006 Nov 2;25(52):6986-96
Versican-thrombospondin-1 binding in vitro and colocalization in microfibrils induced by inflammation on vascular smooth muscle cells.
Kuznetsova SA, Issa P, Perruccio EM, Zeng B, Sipes JM, Ward Y, Seyfried NT, Fielder HL, Day AJ, Wight TN, Roberts DD
Journal of cell science 2006 Nov 1;119(Pt 21):4499-509
Journal of cell science 2006 Nov 1;119(Pt 21):4499-509
Enhanced proangiogenic signaling in thrombospondin-1-deficient retinal endothelial cells.
Wang Y, Wang S, Sheibani N
Microvascular research 2006 May;71(3):143-51
Microvascular research 2006 May;71(3):143-51
Islet endothelial cells and pancreatic beta-cell proliferation: studies in vitro and during pregnancy in adult rats.
Johansson M, Mattsson G, Andersson A, Jansson L, Carlsson PO
Endocrinology 2006 May;147(5):2315-24
Endocrinology 2006 May;147(5):2315-24
Thrombospondins deployed by thrombopoietic cells determine angiogenic switch and extent of revascularization.
Kopp HG, Hooper AT, Broekman MJ, Avecilla ST, Petit I, Luo M, Milde T, Ramos CA, Zhang F, Kopp T, Bornstein P, Jin DK, Marcus AJ, Rafii S
The Journal of clinical investigation 2006 Dec;116(12):3277-91
The Journal of clinical investigation 2006 Dec;116(12):3277-91
Receptor for advanced glycation end products is involved in impaired angiogenic response in diabetes.
Shoji T, Koyama H, Morioka T, Tanaka S, Kizu A, Motoyama K, Mori K, Fukumoto S, Shioi A, Shimogaito N, Takeuchi M, Yamamoto Y, Yonekura H, Yamamoto H, Nishizawa Y
Diabetes 2006 Aug;55(8):2245-55
Diabetes 2006 Aug;55(8):2245-55
Thrombospondins, metallo proteases and thrombospondin receptors messenger RNA and protein expression in different tumour sublines of the Dunning prostate cancer model.
Vallbo C, Damber JE
Acta oncologica (Stockholm, Sweden) 2005;44(3):293-8
Acta oncologica (Stockholm, Sweden) 2005;44(3):293-8
Expression of thrombospondin-1 in resected colorectal liver metastases predicts poor prognosis.
Sutton CD, O'Byrne K, Goddard JC, Marshall LJ, Jones L, Garcea G, Dennison AR, Poston G, Lloyd DM, Berry DP
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 15;11(18):6567-73
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 15;11(18):6567-73
1,25-dihydroxyvitamin D inhibits renal interstitial myofibroblast activation by inducing hepatocyte growth factor expression.
Li Y, Spataro BC, Yang J, Dai C, Liu Y
Kidney international 2005 Oct;68(4):1500-10
Kidney international 2005 Oct;68(4):1500-10
Different involvement of extracellular matrix components in small and large arteries during chronic NO synthase inhibition.
Bouvet C, Gilbert LA, Girardot D, deBlois D, Moreau P
Hypertension (Dallas, Tex. : 1979) 2005 Mar;45(3):432-7
Hypertension (Dallas, Tex. : 1979) 2005 Mar;45(3):432-7
Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling.
Olson LE, Zhang J, Taylor H, Rose DW, Rosenfeld MG
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 8;102(10):3708-13
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 8;102(10):3708-13
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, Einheber S, Boudreau N, Nishimura SL
The American journal of pathology 2005 Jun;166(6):1883-94
The American journal of pathology 2005 Jun;166(6):1883-94
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, Einheber S, Boudreau N, Nishimura SL
The American journal of pathology 2005 Jun;166(6):1883-94
The American journal of pathology 2005 Jun;166(6):1883-94
Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction.
Li SS, Forslöw A, Sundqvist KG
Journal of immunology (Baltimore, Md. : 1950) 2005 Jan 15;174(2):654-61
Journal of immunology (Baltimore, Md. : 1950) 2005 Jan 15;174(2):654-61
Wnt signaling can repress thrombospondin-1 expression in colonic tumorigenesis.
Jo WS, Mizukami Y, Duerr EM, Zukerberg LR, Chung DC
Cancer biology & therapy 2005 Dec;4(12):1361-6
Cancer biology & therapy 2005 Dec;4(12):1361-6
Kinetics of biglycan, decorin and thrombospondin-1 in mercuric chloride-induced renal tubulointerstitial fibrosis.
Suzuki K, Wang R, Kubota H, Shibuya H, Saegusa J, Sato T
Experimental and molecular pathology 2005 Aug;79(1):68-73
Experimental and molecular pathology 2005 Aug;79(1):68-73
Isolation and characterization of murine retinal astrocytes.
Scheef E, Wang S, Sorenson CM, Sheibani N
Molecular vision 2005 Aug 22;11:613-24
Molecular vision 2005 Aug 22;11:613-24
Endogenous thrombospondin-1 is not necessary for proliferation but is permissive for vascular smooth muscle cell responses to platelet-derived growth factor.
Isenberg JS, Calzada MJ, Zhou L, Guo N, Lawler J, Wang XQ, Frazier WA, Roberts DD
Matrix biology : journal of the International Society for Matrix Biology 2005 Apr;24(2):110-23
Matrix biology : journal of the International Society for Matrix Biology 2005 Apr;24(2):110-23
Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice.
Uno K, Kuroki M, Hayashi H, Uchida H, Kuroki M, Oshima K
Histology and histopathology 2005 Apr;20(2):493-9
Histology and histopathology 2005 Apr;20(2):493-9
Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice.
Uno K, Kuroki M, Hayashi H, Uchida H, Kuroki M, Oshima K
Histology and histopathology 2005 Apr;20(2):493-9
Histology and histopathology 2005 Apr;20(2):493-9
Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats.
Ihara Y, Manabe S, Kanda M, Kawano H, Nakayama T, Sekine I, Kondo T, Ito Y
Glycobiology 2005 Apr;15(4):383-92
Glycobiology 2005 Apr;15(4):383-92
Role of thrombospondin-1 in the autologous phase of an accelerated model of anti-glomerular basement membrane glomerulonephritis.
Hochegger K, Knight S, Hugo C, Mayer G, Lawler J, Mayadas TN, Rosenkranz AR
Nephron. Experimental nephrology 2004;96(2):e31-8
Nephron. Experimental nephrology 2004;96(2):e31-8
Thrombospondin-1 in the trabecular meshwork: localization in normal and glaucomatous eyes, and induction by TGF-beta1 and dexamethasone in vitro.
Flügel-Koch C, Ohlmann A, Fuchshofer R, Welge-Lüssen U, Tamm ER
Experimental eye research 2004 Nov;79(5):649-63
Experimental eye research 2004 Nov;79(5):649-63
Thombospondin-1 disrupts estrogen-induced endothelial cell proliferation and migration and its expression is suppressed by estradiol.
Sengupta K, Banerjee S, Saxena NK, Banerjee SK
Molecular cancer research : MCR 2004 Mar;2(3):150-8
Molecular cancer research : MCR 2004 Mar;2(3):150-8
Thrombospondin-1 accelerates wound healing of corneal epithelia.
Uno K, Hayashi H, Kuroki M, Uchida H, Yamauchi Y, Kuroki M, Oshima K
Biochemical and biophysical research communications 2004 Mar 19;315(4):928-34
Biochemical and biophysical research communications 2004 Mar 19;315(4):928-34
Alterations in cell-adhesive and migratory properties of proximal tubule and collecting duct cells from bcl-2 -/- mice.
Ziehr J, Sheibani N, Sorenson CM
American journal of physiology. Renal physiology 2004 Dec;287(6):F1154-63
American journal of physiology. Renal physiology 2004 Dec;287(6):F1154-63
Significance of CD 105 expression for tumour angiogenesis and prognosis in endometrial carcinomas.
Salvesen HB, Gulluoglu MG, Stefansson I, Akslen LA
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 Nov;111(11):1011-8
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 Nov;111(11):1011-8
Significance of CD 105 expression for tumour angiogenesis and prognosis in endometrial carcinomas.
Salvesen HB, Gulluoglu MG, Stefansson I, Akslen LA
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 Nov;111(11):1011-8
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 Nov;111(11):1011-8
Isolation and characterization of murine retinal endothelial cells.
Su X, Sorenson CM, Sheibani N
Molecular vision 2003 May 1;9:171-8
Molecular vision 2003 May 1;9:171-8
Ras modulates Myc activity to repress thrombospondin-1 expression and increase tumor angiogenesis.
Watnick RS, Cheng YN, Rangarajan A, Ince TA, Weinberg RA
Cancer cell 2003 Mar;3(3):219-31
Cancer cell 2003 Mar;3(3):219-31
Connexin26 regulates the expression of angiogenesis-related genes in human breast tumor cells by both GJIC-dependent and -independent mechanisms.
Qin H, Shao Q, Thomas T, Kalra J, Alaoui-Jamali MA, Laird DW
Cell communication & adhesion 2003 Jul-Dec;10(4-6):387-93
Cell communication & adhesion 2003 Jul-Dec;10(4-6):387-93
Connexin26 regulates the expression of angiogenesis-related genes in human breast tumor cells by both GJIC-dependent and -independent mechanisms.
Qin H, Shao Q, Thomas T, Kalra J, Alaoui-Jamali MA, Laird DW
Cell communication & adhesion 2003 Jul-Dec;10(4-6):387-93
Cell communication & adhesion 2003 Jul-Dec;10(4-6):387-93
Regulation of multiple tumor microenvironment markers by overexpression of single or paired combinations of ErbB receptors.
Alaoui-Jamali MA, Song DJ, Benlimame N, Yen L, Deng X, Hernandez-Perez M, Wang T
Cancer research 2003 Jul 1;63(13):3764-74
Cancer research 2003 Jul 1;63(13):3764-74
Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat.
Stenina OI, Krukovets I, Wang K, Zhou Z, Forudi F, Penn MS, Topol EJ, Plow EF
Circulation 2003 Jul 1;107(25):3209-15
Circulation 2003 Jul 1;107(25):3209-15
Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells.
Liu X, Wu Z, Sheibani N, Brandt CR, Polansky JR, Kaufman PL
Experimental eye research 2003 Aug;77(2):181-8
Experimental eye research 2003 Aug;77(2):181-8
Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells.
Liu X, Wu Z, Sheibani N, Brandt CR, Polansky JR, Kaufman PL
Experimental eye research 2003 Aug;77(2):181-8
Experimental eye research 2003 Aug;77(2):181-8
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
Lopes N, Gregg D, Vasudevan S, Hassanain H, Goldschmidt-Clermont P, Kovacic H
Molecular and cellular biology 2003 Aug;23(15):5401-8
Molecular and cellular biology 2003 Aug;23(15):5401-8
Characteristics of long-term survivors of untreated lung cancer.
Mattern J, Koomägi R, Volm M
Lung cancer (Amsterdam, Netherlands) 2002 Jun;36(3):277-82
Lung cancer (Amsterdam, Netherlands) 2002 Jun;36(3):277-82
Characteristics of long-term survivors of untreated lung cancer.
Mattern J, Koomägi R, Volm M
Lung cancer (Amsterdam, Netherlands) 2002 Jun;36(3):277-82
Lung cancer (Amsterdam, Netherlands) 2002 Jun;36(3):277-82
Investigation of the role of nitric oxide synthase 2 in pregnancy using mutant mice.
Burnett TG, Tash JS, Hunt JS
Reproduction (Cambridge, England) 2002 Jul;124(1):49-57
Reproduction (Cambridge, England) 2002 Jul;124(1):49-57
Investigation of the role of nitric oxide synthase 2 in pregnancy using mutant mice.
Burnett TG, Tash JS, Hunt JS
Reproduction (Cambridge, England) 2002 Jul;124(1):49-57
Reproduction (Cambridge, England) 2002 Jul;124(1):49-57
In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin.
Filla MS, Liu X, Nguyen TD, Polansky JR, Brandt CR, Kaufman PL, Peters DM
Investigative ophthalmology & visual science 2002 Jan;43(1):151-61
Investigative ophthalmology & visual science 2002 Jan;43(1):151-61
Overexpression of thrombospondin-1 reduces growth and vascular index but not perfusion in glioblastoma.
Kragh M, Quistorff B, Tenan M, Van Meir EG, Kristjansen PE
Cancer research 2002 Feb 15;62(4):1191-5
Cancer research 2002 Feb 15;62(4):1191-5
Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast.
Rice AJ, Steward MA, Quinn CM
Journal of clinical pathology 2002 Dec;55(12):921-5
Journal of clinical pathology 2002 Dec;55(12):921-5
Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast.
Rice AJ, Steward MA, Quinn CM
Journal of clinical pathology 2002 Dec;55(12):921-5
Journal of clinical pathology 2002 Dec;55(12):921-5
T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components.
Li SS, Ivanoff A, Bergström SE, Sandström A, Christensson B, van Nerven J, Holgersson J, Hauzenberger D, Arencibia I, Sundqvist KG
European journal of immunology 2002 Apr;32(4):1069-79
European journal of immunology 2002 Apr;32(4):1069-79
Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts.
Rofstad EK, Graff BA
The Journal of investigative dermatology 2001 Nov;117(5):1042-9
The Journal of investigative dermatology 2001 Nov;117(5):1042-9
Association of thrombospondin-1 and cardiac allograft vasculopathy in human cardiac allografts.
Zhao XM, Hu Y, Miller GG, Mitchell RN, Libby P
Circulation 2001 Jan 30;103(4):525-31
Circulation 2001 Jan 30;103(4):525-31
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Shi SR, Cote RJ, Hawes D, Thu S, Shi Y, Young LL, Taylor CR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Shi SR, Cote RJ, Hawes D, Thu S, Shi Y, Young LL, Taylor CR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
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- Western blot of Thrombospondin using Thrombospondin Monoclonal Antibody (Product # MA5-13398) on HUVEC Cells.
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- Immunofluorescent analysis of Thrombospondin (green) showing staining in the secretion of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thrombospondin monoclonal antibody (Product # MA5-13398) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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- Immunofluorescent analysis of Thrombospondin (green) showing staining in the secretion of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thrombospondin monoclonal antibody (Product # MA5-13398) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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- Immunofluorescent analysis of Thrombospondin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thrombospondin monoclonal antibody (Product # MA5-13398) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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- Formalin-fixed, paraffin-embedded human placenta stained with Thrombospondin antibody using peroxidase-conjugate and AEC chromogen. Note staining of platelets in the blood vessels.
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- Immunohistochemistry analysis of Thrombospondin showing positive staining in the Extracellular and platelet A-granules of paraffin-treated Human placenta tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thrombospondin monoclonal antibody (Product # MA5-13398) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Immunohistochemistry analysis of Thrombospondin showing positive staining in the Extracellular and platelet A-granules of paraffin-treated Human tonsil tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thrombospondin monoclonal antibody (Product # MA5-13398) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Immunohistochemistry analysis of Thrombospondin showing positive staining in the Extracellular and platelet A-granules of paraffin-treated Mouse lung tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Thrombospondin monoclonal antibody (Product # MA5-13398) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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- Flow cytometry analysis of Thrombospondin in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Thrombospondin monoclonal antibody (Product # MA5-13398) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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- Flow cytometry analysis of Thrombospondin in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Thrombospondin monoclonal antibody (Product # MA5-13398) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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- Flow cytometry analysis of Thrombospondin in HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Thrombospondin monoclonal antibody (Product # MA5-13398) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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- Flow cytometry analysis of Thrombospondin in L929 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Thrombospondin monoclonal antibody (Product # MA5-13398) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
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- Immunoprecipitation of Thrombospondin using Thrombospondin Monoclonal Antibody (Product # MA5-13398) on Native Human HUVEC Cells.
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- FIGURE 5. Efb interacts with platelet P-selectin. A , human platelets were ultrasonicated in the presence or absence of 3 mg/ml added fibrinogen. Sigma His-Select HF nickel affinity gel was used to capture His-tagged Efb- or Efb-N-interacting proteins from platelet lysates. Affinity precipitates were separated by SDS-PAGE and immunoblotted for integrin alphaIIb, P-selectin, multimerin-1, fibronectin, thrombospondin-1 (indicated by Thrombosp.-1 ), and His tag (to confirm Efb/Efb-N expression and immobilization). Immunoblots shown here are representative of those from three independent experiments. -ve , negative control; PD , pulldown. B , SPR data confirm the direct interaction of Efb and P-selectin. Recombinant P-selectin-Fc immobilized on COOHV-coated SensiQ TM sensor chips was treated with increasing concentrations of Efb (100 n m ( brown ), 300 n m ( purple ), and 2.8 mu m ( green )). All experiments were performed using the SensiQ TM Pioneer FE SPR platform (SensiQ Technologies). Experimental data were fit using the Qdat data analysis software. Curves were fitted using a least squares fitting algorithm. RU , response units.
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- Figure 3 Analysis of miRNA targets. (A) Venn diagram summarizing the rationale for selecting putative miRNA targets by combining the lists generated with prediction algorithms with those generated from expression datasets (1,858 genes with a fold change
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- Fig 3 Alterations to VEGF-A and TSP-1 with elevated CORT and concomitant prazosin treatment. RNA or protein was isolated from the TA muscle after 1W or 2W of CORT with or without concurrent prazosin treatment. Taqman qPCR was used to assess the mRNA levels of VEGF-A (A,B) and TSP-1 (D,E), while VEGF-A protein was assessed by ELISA (C) and TSP-1 protein by Western blot (F). (A) VEGF-A mRNA was not altered in response to 1W CORT and/or prazosin. (B) VEGF-A mRNA was not affected by 2W CORT ( P = 0.08), while a significant prazosin effect was detected within the 2W CORT-prazosin group (* P
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- Figure 3 High glucose treatment shifted endothelial cell angiomiR expression towards an anti-angiogenic phenotype. ( A ) Primary miR and ( B ) mature miR levels relative to HPRT housekeeping gene and exogenous spike-in cel-miR-39-3p, respectively. Pro-angiogenic pri-miRs and miR are underlined in red, while angiostatic pri-miRs and miRs are underlined in blue. Data are means +- SEM ( n = 6). Two-way ANOVA showed no effect of glucose concentration on the expression of pri-miR-126, pri-miR-17-92, and pri-miR-15; *** indicates significative difference compared to 5 mM of glucose ( p < 0.001)). ( C , D ) VEGF-A and THBS-1 mRNA relative to HPRT in HDMEC treated with 5 or 30 mM glucose concentrations for 24 h (data are means +- SEM, n = 9). ( E , F ) Immunoblots of miR targets VEGF-A and THBS-1 (A6.1) after 24 h treatment under 5 or 30 mM glucose conditions. alpha,beta-TUBULIN was used as a loading control. Data are means +- SEM; * indicates significative differences ( p < 0.05, t -test, n = 5-10).
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- Figure 6 MT 1- MMP absence in endothelial cells leads to perivascular accumulation of thrombospondin-1 ( TSP 1) in the inflamed colon A, B Representative maximum-intensity projection images of staining for TSP1 (red) and CD31 (gray) in (A) and also for MT1-MMP (green) in (B) in colon sections obtained from MT1 f/f and MT1 iDeltaEC mice left untreated (A) or treated with 1% DSS for 3 days (B). Magnified views are shown to the right. Scale bars, 100 mum in main panels and 10 mum in the magnified views. C TSP1 mean fluorescence intensity (MFI) in the perivascular area of large vessels present in the colon mucosa of treated mice, as depicted in (B); n = 4 mice per genotype. Data are shown as mean +- SEM and were tested by t -test; * P < 0.05. Data information: Please see Appendix Table S3 for exact P -values. Source data are available online for this figure.
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- Figure 2 MTRN localization in non-human primate retina flat-mount. Immunostaining on the flat-mount retina of MTRN (green), TSP1 (red), and GS (red). MTRN and TSP1 are highly concentrated around the optic nerve head (NO = Optic Nerve), and co-localize at the macula and around the fovea* ( a ). Colocalization of MTRN with GS expressed in Muller cells at the macula ( b and inset).
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- Figure 4 Human immunostaining of MTRN and TSP1 on retinal cryosection. Immunostaining on human retinal cryosection of MTRN (green), TSP1 (red), counter marked with DAPI (blue). MTRN (green) colocalized in all layers with TSP1, in macula ( a ) and is expressed in the cytoplasm and in nuclei of ganglion cells, in nuclei of inner and outer layers (INL, ONL) (b), in Muller fibers ( c ), and in the inner segment of photoreceptors ( d ). Close to the optic nerve, MTRN is strongly present in nerves fibers ( e ), in Bruch's membrane (BM) ( f ). MTRN is also found as a gradient starting from the BM towards the deeper choroidal vessels ( f ).
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- Figure 7 MTRN effect on CNV quantified by isolectin B4. Choroidal flat-mount laser impact images, for each treatment, stained with ss4-isolectin (green) ( a ). Quantification of neovascular volume ( b ). Immunostaining of isoLB4 (green), GFAP (red), and TSP1 (purple) on neuroretina treated by PBS ( c , d and insets) and MTRN ( g , h and insets), and on laser impacts of RPE-choroid complex treated by PBS ( e , f ) and MTRN ( i,j ). * ( p = 0.03), ** ( p < 0.01), *** ( p < 0.001).
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- Figure 5 Super-resolution images of secretory vesicles in activated platelets. ( a ) 2D STORM images of alpha-granules in the activated platelet. alpha-granules were observed from the platelets that were fixed at different activation time points (0, 5, 10, 15, and 20 min) (yellow arrows: centralization of alpha-granules upon activation). ( b ) The radial distribution graph of alpha-granules showing how alpha-granules-positive pixel density varies as a function of distance from the centroid of the platelet is shown in ( a ), implying the centralization of alpha-granules upon activation. The vacant space in the center was observed at 10-15 min, probably suggesting OCS regions. ( c ) The bar graph indicates the number of alpha-granules per platelet upon activation induced by PMA (mean +- SD; n = 10-23). ( d ) The bar graph indicates the size of alpha-granules upon activation induced by PMA (mean +- SD; n = 19-28). ( e ) The bar graph indicates the averaged alpha-granule-positive pixel number per platelet upon activation induced by PMA (mean +- SD; n = 21). ( f ) 2D STORM images of dense granules in the activated platelet. Dense granules were observed from the platelets that were fixed at different activation time points (0, 5, 10, 15, and 20 min) (yellow arrows: centralization of dense granules upon activation). ( g ) The radial distribution graph of dense granules showing how dense granules-positive pixel density varies as a function of distance from the centroid of the platele
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- Fig. 6 Downregulation of ECM factors in CEBPB -ko cells. a Expression of ECM-associated genes relative to GAPDH in wt BT-20 cells and two different clones of CEBPB -ko BT-20 cells ( n = 3). Results are analysed using one-way ANOVA analysis. Error bars represent SD, ** p < 0.01, **** p < 0.0001. b C/EBPbeta-associated fragments associated with H3K acetylation (H3K27Ac). Data from the ENCODE database ( http://genome.ucsc.edu/ENCODE/ )(see Supplementary Table 1 ). c Immunoblots showing expression of Thrombospondin 1 (THBS1), Tenascin C (TNC), Fibronectin 1 (FN1) or Collagen 5A1 (COL5A1) in wt BT-20 cells and two different clones of Cebpb -ko BT-20 cells. Loss of C/EBPbeta-LAP expression shows the successful CEBPB -knockout and beta-actin was used for loading control.
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- Isolation and characterization of human corneal keratocyte (HCK)-, fibroblast (HCF)-, and myofibroblast (HCM)-derived extracellular vesicles (EVs). ( A ) EV pellets (20 mug protein/lane) were analyzed by western blot to probe for vesicle-associated markers. Representative images are shown for vesicle-associated proteins CD63, CD81, and ITGAV; a negative control (GM130); and proteins associated with corneal wound healing, FN1 and THBS1. ( B ) EV pellets were analyzed by nanoparticle tracking analysis (NTA), and a size distribution histogram for each EV sample is shown. (( B ), inset) Transmission electron microscopy images demonstrating EV morphology (high magnification, 49000x; scale bar = 100 nm). ( C ) The average particle concentrations (particles x1011/mL); ( D ) mean particle size (nm); and ( E ) zeta (zeta) potential of EV pellets were measured using NTA with Zetaview(tm). Data are shown as mean + SEM; n = 3 independent EV preparations; ns = nonsignificant. One-way ANOVA with Tukey's post-test.
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- Changes in TSP1 and TSP2 protein expressoin following hemorrhage injury. (A) Representative western blots of TSP1 protein expression and (B) semi-quantification of the band density in the sham group and at each time point after modeling. (C) Representative western blots of TSP2 protein expression and (D) semi-quantification of the band density in the sham group and at each time point after modeling. * P
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- 2 The effects of PLK1 downregulation in EC cells. siPLK1 (siPLK286, siPLK785 and siPLK1412) or siNTC was transfected into EC cells. LF- and LF+ represent no transfection and transfection with Lipofectamine RNAiMAX alone, respectively. ( a ) Cell proliferation assay after PLK1-siRNA transfection. HSA/c, KYSE140, KYSE410 and OE33 cells were subjected to WST-8 assay 48 hr after the transfection. The absorbances at 450 nm were measured after 2-hr incubation with WST-8 reagent at 2, 4 and 6 days after plating cells. p values were calculated by two-way ANOVA. ( b ) Cell cycle analysis after PLK1-siRNA transfection. HSA/c and OE33 cells were stained with propidium iodide and analyzed by flow cytometry 48 hr after the siPLK1 transfection. Average percentages of cells in G2/M phase are shown in the representative histograms. ( c ) Localization of PLK1 in siRNA-treated HSA/c cells (magnification x200). HSA/c cells were stained with PLK1, which was labeled with AlexaFluor 568 (red), and nuclei were counterstained with 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI, blue) 48 hr after the siRNA transfection. Left, cells stained with PLK1; middle, cells stained with DAPI; right, merged images. Top, cells transfected with siNTC; bottom, cells transfected with siPLK286. Photographs were taken for identical exposure intervals (70 msec for PLK1 and 3 msec for DAPI). PLK1 was localized mainly to the nuclei of mitotic cells, particularly at the spindle poles from prophase to metaphase (yel
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- Figure 3 High expression of disulphide-HMGB1 in human leukocytes. (A) Western blot probed with anti-CD45, anti-HMGB1, and anti-GAPDH antibodies in reducing conditions (upper panels) or probed with anti-HMGB1 antibody in non-reducing conditions (lower panel) on peripheral blood mononuclear cells (PBMCs) isolated from four healthy human donors. The upper band corresponds to the fully reduced-HMGB1 (frHMGB1) and the lower band to the disulphide-HMGB1 (dsHMGB1) in the lower panel. (B) Quantification of HMGB1 redox isoforms expression normalized on Ponceau staining. A.U. = arbitrary unit ( n = 4 healthy donors). (C,D) Western blot probed with anti-HMGB1 antibody in non-reducing conditions on PBMCs stimulated with anti-CD3/anti-CD28 antibodies or lipopolysaccharide (LPS) for 24 or 72 h (C) . Percentage of HMGB1 redox isoforms expression (D) . Ctrl, control unstimulated cells ( n = 2 healthy donors). Data represent the means +- SEM and statistical significance was calculated by Two-way ANOVA (D) .
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- Figure 1 Overexpression of SETDB1 in Melanoma Cell Lines Resulted in the Activation of Protumorigenic-Secreted Factors (A) Histogram plot showing microarray expression data (shown as log 2 fold change) of genes encoding for protumorigenic-secreted factors (THBS1, SCG2, MMP1, MMP3, IL-6, IL-8, CCL2, and WNT5A) in HT 144-SETDB1 OE cells compared to EV cells. (B) Quantitative real-time PCR analysis of the CRS factor-encoding genes SCG2 , MMP3 , IL8 , CCL2 , and WNT5A expression 5. (C) Secretome analysis of HT 144 EV and -SETDB1 OE cell supernatant. Right panel: blots showing the signals of 55 different secreted proteins detected in the supernatant of cultured cells; signals of MMP8 (1), CXCL16 (2), CCL2 (3), and THBS1 (4) are highlighted. Left panel: quantification of mean pixel density related to MMP8, CXCL16, CCL2, and THBS1 signals. Number or replicates: 3. (D) SCG2 immunofluorescence detection in C32 EV (control) and -SCG2 OE cell lines. SCG2-overexpressing cells showed a strong SCG2 signal accumulation at granule structures (indicated by arrows). DAPI stained the nuclei. Scale bars, 20 mum. (E) Tissue microarray analysis (TMA) of a cohort of melanoma patients showing that SETDB1 expression positively correlated with SCG2 expression in melanoma metastases tissue samples. SETDB1 and SCG2 correlation plot is shown. Number of analyzed patients' samples (n), Spearman correlation coefficient (r), and p value are reported. (F) Kaplan-Meier survival analysis of patients with melano