Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-35960 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPH3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: mouse brain, rat brain. Predicted reactivity: Mouse (88%), Rat (88%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPH3A was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RPH3A Polyclonal Antibody (Product # PA5-35960) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPH3A Polyclonal Antibody (Product # PA5-35960). Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RPH3A Polyclonal Antibody (Product # PA5-35960) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RPH3A was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RPH3A Polyclonal Antibody (Product # PA5-35960) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RPH3A was performed in paraffin-embedded rat brain tissue using RPH3A Polyclonal Antibody (Product # PA5-35960) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPH3A Polyclonal Antibody detects Rabphilin 3A protein at membrane on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. RPH3A Polyclonal Antibody (Product # PA5-35960) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.