Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-80711 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGFBP7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGFBP7 in Lane A: THP1 Whole Cell Lysate (30 µg), Lane B: Rat brain tissue Lysate (30 µg), Lane C: Mouse kidney tissue Lysate (30 µg), . Samples were probed using a IGFBP7 Polyclonal Antibody (Product # PA5-80711) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:29 kDa. Observed band size:29 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IGFBP7 Polyclonal Antibody (Product # PA5-80711) at 1:500 dilution. Lane A: THP1 Whole Cell Lysate, Lane B: Rat brain tissue lysate, Lane C: Mouse kidney tissue lysate. Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGFBP7 Immunoprecipitation using: Lane A: 0.5 mg THP-1 Whole Cell Lysate 1 µL with IGFBP7 Polyclonal Antibody (Product # PA5-80711) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: IGFBP7 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.