MA5-15700

antibody from Invitrogen Antibodies

Targeting: KLHL1 FLJ30047, KIAA1490, MRP2

ELISA

Antibody data

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Validation data

Product number: MA5-15700 - Provider product page
Provider: Invitrogen Antibodies
Product name: KLHL1 Monoclonal Antibody (3A8)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15700 targets KLHL1 in indirect ELISA, IF and WB applications and shows reactivity with Human, Mouse and Rat samples. The MA5-15700 immunogen is purified recombinant fragment of human KLHL1 expressed in E. Coli. MA5-15700 detects KLHL1 which has a predicted molecular weight of approximately 82kDa. In Western blot, MA5-15700 did not detect KLHL1 in whole rat brain lysates, but detected KLHL1 in hippocampal neuronal cell lysates.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 3A8
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

KLHL1 protein structure - MA5-15700 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLHL1 was performed by loading 75 µg or 100 µg mouse (Ms) and rat (Rt) whole brain lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked for 1 hour with TBST + 5% milk. The membrane was probed with a KLHL1 monoclonal antibody (Product # MA5-15700) at a dilution of 1:10,000 overnight at 16C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:2500 for 1 hour at room temperature. Detection was performed using SuperSignal West Femto (Product # 34095). Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLHL1 was performed by loading 100 µg of rat hippocampal neuronal culture lysate per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked for 1 hour with TBST + 5% milk. The membrane was probed with a KLHL1 monoclonal antibody (Product # MA5-15700) at a dilution of 1:10,000 overnight at 16C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:2500 for 1 hour at room temperature. Detection was performed using SuperSignal West Femto (Product # 34095). Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLHL1 using a KLHL1 monoclonal antibody (Product # MA5-15700) against a human KLHL1 (AA: 202-506) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLHL1 using a KLHL1 monoclonal antibody (Product # MA5-15700) against a human KLHL1 (AA: 202-506) recombinant protein.