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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502709 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502709, RRID:AB_11125366
- Product name
- PDLIM2 mouse monoclonal antibody, clone OTI1D12 (formerly 1D12)
- Antibody type
- Monoclonal
- Description
- PDLIM2 mouse monoclonal antibody, clone OTI1D12 (formerly 1D12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDLIM2
- Isotype
- IgG
- Antibody clone number
- OTI1D12
- Vial size
- 100 µl
- Concentration
- 0.68 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM2 (RC210022, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PDLIM2 mouse monoclonal antibody (TA502709) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDLIM2(RC210022).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDLIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502709)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PDLIM2 antibody(TA502709),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC210022 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDLIM2 antibody(TA502709), and then analyzed by flow cytometry.
- Validation comment
- FC
