Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-53207 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC25A22 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: RSEGYFGMYR GAAVNLTLVT PEKAIKLAAN DFFRHQLSKD GQKLTLLKEM LAGCGAGTCQ VIVTTPMEML KIQLQDAGRI AAQRKILAAQ GQLSAQGGAQ PSVE Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 94%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A22 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a SLC25A22 Polyclonal Antibody (Product # PA5-53207).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC25A22 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a SLC25A22 Polyclonal Antibody (Product # PA5-53207).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SLC25A22 in human cell line A-431 shows positivity in mitochondria & nucleus but excluded from the nucleoli. Samples were probed using a SLC25A22 Polyclonal Antibody (Product # PA5-53207).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SLC25A22 in human skeletal muscle using SLC25A22 Polyclonal Antibody (Product # PA5-53207) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SLC25A22 in human testis using SLC25A22 Polyclonal Antibody (Product # PA5-53207) shows strong granular positivity in cytoplasm in a subset of cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SLC25A22 in human prostate using SLC25A22 Polyclonal Antibody (Product # PA5-53207) shows strong granular positivity in cytoplasm in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SLC25A22 in human cerebral cortex using SLC25A22 Polyclonal Antibody (Product # PA5-53207) shows strong granular positivity in cytoplasm in neurons.