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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA506986 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA506986, RRID:AB_2624024
- Product name
- MADCAM1 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)
- Antibody type
- Monoclonal
- Description
- MADCAM1 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MADCAM1
- Isotype
- IgG
- Antibody clone number
- OTI1E1
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-MADCAM1 mouse monoclonal antibody (TA506986) at 1:100 dilution.
- Validation comment
- IF
