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- Antibody Data
- Antigen structure
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- Product number
- MAB60561-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MAdCAM-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human MADCAM-1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13477- Isotype
- IgG
- Antibody clone number
- 683723R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Human MAdCAM-1 ELISA Standard Curve. Recombinant Human MAdCAM-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human MAdCAM-1 Monoclonal Antibody (Catalog # MAB60561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human MAdCAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6056) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
