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LearnPA5-77280
antibody from Invitrogen Antibodies
Targeting: AGTR1
AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-77280 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AGTR1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes with AGTR1 (extracellular) polyclonal antibody (Product # PA5-77280) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes with AGTR1 (extracellular) polyclonal antibody (Product # PA5-77280) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes with AGTR1 (extracellular) polyclonal antibody (Product # PA5-77280) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of AGTR1 (extracellular) in rat C6 glioma cells. A) Samples were probed with AGTR1 (extracellular) polyclonal antibody (Product # PA5-77280) at a dilution of 1:100, and incubated with goat anti-rabbit-AlexaFluor-555. B) Live view cell image.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AGTR1 (extracellular) in mouse cerebellum. Samples were probed with AGTR1 (extracellular) polyclonal antibody (Product # PA5-77280). A) Stained Purkinje layer (red). B) Stained AT receptors. C) Merged images from Panel A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Jurkat cells using polyclonal AGTR1 (extracellular) antibody (Product # PA5-77280) with a dilution of 10 µg followed by a goat anti-rabbit FITC secondary antibody.
