H00006197-D01P

antibody from Abnova Corporation

Targeting: RPS6KA3 CLS, HU-3, MRX19, RSK2

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006197-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006197-D01P, RRID:AB_1709951

Product name

RPS6KA3 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RPS6KA3 protein.

Antigen sequence

MPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGE
EEINPQTEEVSIKEIAITHHVKEGHEKADPSQFEL
LKVLGQGSFGKVFLVKKILGSDARQLYAMKVLKKA
TLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTE
GKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLA
ELALALDHLHSLGIIYRDLKPENILLDEEGHIKLT
DFGLSKESIDHEKKAYSFCGTVEYMAPEVVNRRGH
TQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMI
LKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGAGP
DGVEEIKRHSFFSTIDWNKLYRREIHPPFKPATGR
PEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGF
SFVAITSDDESQAMQTVGVHSIVQQLHRNSIQFTD
GYEVKEDIGVGSYSVCKRCIHKATNMEFAVKIIDK
SKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVY
VVTELMKGGELLDKILRQKFFSEREASAVLFTITK
TVEYLHAQGVVHRDLKPSNILYVDESGNPESIRIC
DFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGY
DAACDIWSLGVLLYTMLTGYTPFANGPDDTPEEIL
ARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPH
QRLTAALVLRHPWIVHWDQLPQYQLNRQDAPHLVK
GAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKI
TSTAL


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RPS6KA3 MaxPab rabbit polyclonal antibody. Western Blot analysis of RPS6KA3 expression in human placenta.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RPS6KA3 MaxPab rabbit polyclonal antibody. Western Blot analysis of RPS6KA3 expression in mouse lung.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RPS6KA3 expression in transfected 293T cell line (H00006197-T01) by RPS6KA3 MaxPab polyclonal antibody.Lane 1: RPS6KA3 transfected lysate(83.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RPS6KA3 and MAPK1. HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-MAPK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)