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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab126046 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant RSK2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human RSK2 Antibody, Recombinant, could be used for WB, IHC and so on.Application:WB: 1/500 - 1/1000IHC: 1/50-1/100 Protein FunctionSerine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.Post-translationalAutophosphorylated on Ser-386, as part of the activation process.Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant RSK2 Antibody (Ab126046) - Western Blot All lanes: Recombinant RSK2 Antibody (Ab126046) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 84 kDa Observed band size: 89 kDa Exposure time: 2.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant RSK2 Antibody (Ab126046) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RSK2 Antibody (Ab126046). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
