NB300-526

antibody from Novus Biologicals

Targeting: EDN1 ET1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Radioimmunoassay (Data presented on provider website)

Antibody Data

Product number

NB300-526

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-526, RRID:AB_10130596

Product name

Mouse Monoclonal Endothelin-1 Antibody

Antibody type

Monoclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat, Canine, Porcine, Rabbit, Sheep

Host

Mouse

Antigen sequence

Studies suggest that this antibody
binds to an epitope in the region o
f ET-1 represented by amino acids 8
-16.


Isotype

IgG

Vial size

100 uL

Concentration

2.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Decreased Ambient Oxygen Tension Alters the Expression of Endothelin-1, iNOS and cGMP in Rat Alveolar Macrophages.

Chen IC, Lin YT, Huang JS, Wu BN, Hsu JH, Tan MS, Dai ZK
International journal of medical sciences 2019;16(3):443-449

---

NET Formation in Bullous Pemphigoid Patients With Relapse Is Modulated by IL-17 and IL-23 Interplay.

Giusti D, Bini E, Terryn C, Didier K, Le Jan S, Gatouillat G, Durlach A, Nesmond S, Muller C, Bernard P, Antonicelli F, Pham BN
Frontiers in immunology 2019;10:701

---

Endothelin-1 overexpression: a potential biomarker of unfavorable prognosis in non-metastatic muscle-invasive bladder cancer.

Mitrakas L, Gravas S, Karasavvidou F, Dimakopoulos G, Moutzouris G, Tzortzis V, Koukoulis G, Papandreou C, Melekos M
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Jun;36(6):4699-705

---

BMPR2 mutation alters the lung macrophage endothelin-1 cascade in a mouse model and patients with heritable pulmonary artery hypertension.

Talati M, West J, Blackwell TR, Loyd JE, Meyrick B
American journal of physiology. Lung cellular and molecular physiology 2010 Sep;299(3):L363-73

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Analysis of 25 ug of PC12 cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Immunolocalization of ET-1 in human bowel.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Immunolocalization of ET-1 in human bowel.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Analysis of Endothelin 1 in HepG2 cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Analysis of Endothelin 1 in HepG2 cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Analysis of Endothelin 1 in 293T cells compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Analysis of Endothelin 1 in 3T3 cells compared to an isotype control (blue).