AF4277

antibody from R&D Systems

Targeting: REN

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4277

Provider

R&D Systems

Product name

Mouse Renin 1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Renin 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 5% cross-reactivity with recombinant rat Renin is observed, and less than 0.2% cross-reactivity with recombinant human Renin is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P06281


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Transcriptome-based analysis of kidney gene expression changes associated with diabetes in OVE26 mice, in the presence and absence of losartan treatment.

Komers R, Xu B, Fu Y, McClelland A, Kantharidis P, Mittal A, Cohen HT, Cohen DM
PloS one 2014;9(5):e96987

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Chicken ovalbumin upstream promoter transcription factor II regulates renin gene expression.

Mayer S, Roeser M, Lachmann P, Ishii S, Suh JM, Harlander S, Desch M, Brunssen C, Morawietz H, Tsai SY, Tsai MJ, Hohenstein B, Hugo C, Todorov VT
The Journal of biological chemistry 2012 Jul 13;287(29):24483-91

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Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron.

Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F
The Journal of biological chemistry 2010 Dec 31;285(53):41935-46

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Renin in Mouse Kidney. Renin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glomeruli and tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.