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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [2]
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- Product number
- AF4090 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Renin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects both pro and mature forms of human Renin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Renin-1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P60016- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A single-cell survey of the human first-trimester placenta and decidua.
Human kidney pericytes produce renin.
Suryawanshi H, Morozov P, Straus A, Sahasrabudhe N, Max KEA, Garzia A, Kustagi M, Tuschl T, Williams Z
Science advances 2018 Oct;4(10):eaau4788
Science advances 2018 Oct;4(10):eaau4788
Human kidney pericytes produce renin.
Stefanska A, Kenyon C, Christian HC, Buckley C, Shaw I, Mullins JJ, Péault B
Kidney international 2016 Dec;90(6):1251-1261
Kidney international 2016 Dec;90(6):1251-1261
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Renin in Human Kidney. Renin was detected in immersion fixed paraffin-embedded sections of human kidney array using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Renin in Human Kidney. Renin was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
