PB9414
antibody from Boster Biological Technology
Targeting: ABCB11
ABC16, BSEP, PFIC-2, PFIC2, PGY4, SPGP
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9414 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ABCB11 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for BSEP/ABCB11 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. BSEP/ABCB11 information: Molecular Weight: 146407 MW; Subcellular Localization: Membrane; Multi-pass membrane protein; Tissue Specificity: Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Hypercholesterolemia risk associated Abca6 does not regulate lipoprotein metabolism in mice or hamster.
The zonula occludens protein family regulates the hepatic barrier system in the murine liver.
He B, Kang S, Chen Z, Liu X, Wang J, Li X, Liu X, Zheng L, Luo M, Wang Y
Biochimica et biophysica acta. Molecular and cell biology of lipids 2021 Nov;1866(11):159006
Biochimica et biophysica acta. Molecular and cell biology of lipids 2021 Nov;1866(11):159006
The zonula occludens protein family regulates the hepatic barrier system in the murine liver.
Itoh M, Terada M, Sugimoto H
Biochimica et biophysica acta. Molecular basis of disease 2021 Jan 1;1867(1):165994
Biochimica et biophysica acta. Molecular basis of disease 2021 Jan 1;1867(1):165994
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ABCB11 using anti-ABCB11 antibody (PB9414). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate Lane 2: Mouse Liver Tissue Lysate Lane 3: SMMC Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB11 antigen affinity purified polyclonal antibody (Catalog # PB9414) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB11 at approximately 146KD. The expected band size for ABCB11 is at 146KD.
- Additional image