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antibody from Invitrogen Antibodies
Targeting: ABCB11
ABC16, BSEP, PFIC-2, PFIC2, PGY4, SPGP
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- PA5-78690 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BSEP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.
Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis.
Li Z, Chiang YP, He M, Worgall TS, Zhou H, Jiang XC
iScience 2021 Dec 17;24(12):103449
iScience 2021 Dec 17;24(12):103449
Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis.
Li H, Xi Y, Xin X, Tian H, Hu Y
Nutrition & metabolism 2020;17:34
Nutrition & metabolism 2020;17:34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of BSEP in A431 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with BSEP Polyclonal Antibody (Product # PA5-78690) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 11 Changes in FGF15, FGFR4, KLB, CYP7A1, NTCP, and BSEP protein content in liver tissues among the different experimental groups. a Bar chart representing the relative CYP7A1 protein expression level in liver tissue, b relative NTCP protein expression level, c relative BSEP protein expression level, d relative FGF15 protein expression level, e relative FGFR4 protein expression level, and f relative KLB protein expression level in liver tissue. g Representative autoradiograph image of western blotting with the different antibodies. ** P < 0.01, vs control group; ## P < 0.01, # P < 0.05, vs HFD group. Data are presented as mean +- standard deviation, n = 3. C, control; HFD, high-fat diet; Gyp, gypenosides; OCA, obeticholic acid
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 SMS1 deficiency-mediated hepatocyte impairment Liver sections from 6-month-old WT, Sms2 KO, and Sms1 / Sms2 KO female mice were utilized. (A) Na + -taurocholate co-transporting polypeptide (NTCP) immunostaining for hepatocyte basal membrane. (B) Bile salt export pump (BSEP) immunostaining for hepatocyte apical membrane. The pictures are the representatives of four mice/group. (C) Plasma was collected from WT, Sms2 KO, and Sms1 / Sms2 KO mice, and jaundice was observed. (D) Fluorogram of plasma bilirubin (conjugated) levels. (E) Fluorogram of plasma total bile acid levels. (F and G) Fluorogram of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. Data are represented as mean +- SD, n = 4-6, *p < 0.01.
