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LearnPA5-27742
antibody from Invitrogen Antibodies
Targeting: ABCB11
ABC16, BSEP, PFIC-2, PFIC2, PGY4, SPGP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-27742 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ABCB11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat. Predicted reactivity: Mouse (85%), Rat (87%), Dog (84%), Cat (87%), Rabbit (85%), Bovine (88%), Guinea pig (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.17 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Plasmodium vivax liver stage assay platforms using Indian clinical isolates.
Subramani PA, Vartak-Sharma N, Sreekumar S, Mathur P, Nayer B, Dakhore S, Basavanna SK, Kalappa DM, Krishnamurthy RV, Mukhi B, Mishra P, Yoshida N, Ghosh SK, Shandil R, Narayanan S, Campo B, Hasegawa K, Anvikar AR, Valecha N, Sundaramurthy V
Malaria journal 2020 Jun 22;19(1):214
Malaria journal 2020 Jun 22;19(1):214
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ABCB11 Polyclonal Antibody (Product # PA5-27742). Sample (30 µg of whole cell lysate). Lane A: JurKat. 5% SDS PAGE. ABCB11 Polyclonal Antibody (Product # PA5-27742) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ABCB11 Polyclonal Antibody (Product # PA5-27742). Various tissue extracts (5 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ABCB11 Polyclonal Antibody (Product # PA5-27742) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BSEP showing staining in the membrane of A431 cells. A431 cells were fixed in ice-cold MeOH for 5 min and stained using a BSEP polyclonal antibody (Product # PA5-27742) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ABCB11 Polyclonal Antibody detects ABCB11 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ABCB11 protein stained by ABCB11 Polyclonal Antibody (Product # PA5-27742) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded AGS xenograft, using BSEP (Product # PA5-27742) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
