Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB3400 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse cIAP Pan-specific Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse cIAP-1 and cIAP-2 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13489
- Isotype
- IgG
- Antibody clone number
- 315301
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Disruption of XIAP-RIP2 Association Blocks NOD2-Mediated Inflammatory Signaling.
Characterization of Potent SMAC Mimetics that Sensitize Cancer Cells to TNF Family-Induced Apoptosis.
Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model.
IGF2BP1 controls cell death and drug resistance in rhabdomyosarcomas by regulating translation of cIAP1.
TRAIL activates JNK and NF-κB through RIP1-dependent and -independent pathways.
A RIPK3-caspase 8 complex mediates atypical pro-IL-1β processing.
Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-κB activation.
TNFR-associated factor 2 deficiency in B lymphocytes predisposes to chronic lymphocytic leukemia/small lymphocytic lymphoma in mice.
Linear ubiquitination prevents inflammation and regulates immune signalling.
Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-κB pathway.
Goncharov T, Hedayati S, Mulvihill MM, Izrael-Tomasevic A, Zobel K, Jeet S, Fedorova AV, Eidenschenk C, deVoss J, Yu K, Shaw AS, Kirkpatrick DS, Fairbrother WJ, Deshayes K, Vucic D
Molecular cell 2018 Feb 15;69(4):551-565.e7
Molecular cell 2018 Feb 15;69(4):551-565.e7
Characterization of Potent SMAC Mimetics that Sensitize Cancer Cells to TNF Family-Induced Apoptosis.
Welsh K, Milutinovic S, Ardecky RJ, Gonzalez-Lopez M, Ganji SR, Teriete P, Finlay D, Riedl S, Matsuzawa S, Pinilla C, Houghten R, Vuori K, Reed JC, Cosford ND
PloS one 2016;11(9):e0161952
PloS one 2016;11(9):e0161952
Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model.
Rebolleda N, Losada-Fernandez I, Perez-Chacon G, Castejon R, Rosado S, Morado M, Vallejo-Cremades MT, Martinez A, Vargas-Nuñez JA, Perez-Aciego P
PloS one 2016;11(4):e0154159
PloS one 2016;11(4):e0154159
IGF2BP1 controls cell death and drug resistance in rhabdomyosarcomas by regulating translation of cIAP1.
Faye MD, Beug ST, Graber TE, Earl N, Xiang X, Wild B, Langlois S, Michaud J, Cowan KN, Korneluk RG, Holcik M
Oncogene 2015 Mar 19;34(12):1532-41
Oncogene 2015 Mar 19;34(12):1532-41
TRAIL activates JNK and NF-κB through RIP1-dependent and -independent pathways.
Zhang L, Dittmer MR, Blackwell K, Workman LM, Hostager B, Habelhah H
Cellular signalling 2015 Feb;27(2):306-14
Cellular signalling 2015 Feb;27(2):306-14
A RIPK3-caspase 8 complex mediates atypical pro-IL-1β processing.
Moriwaki K, Bertin J, Gough PJ, Chan FK
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 15;194(4):1938-44
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 15;194(4):1938-44
Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-κB activation.
Moreno-García ME, Sommer K, Rincon-Arano H, Brault M, Ninomiya-Tsuji J, Matesic LE, Rawlings DJ
Molecular and cellular biology 2013 Mar;33(6):1149-63
Molecular and cellular biology 2013 Mar;33(6):1149-63
TNFR-associated factor 2 deficiency in B lymphocytes predisposes to chronic lymphocytic leukemia/small lymphocytic lymphoma in mice.
Pérez-Chacón G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 15;189(2):1053-61
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 15;189(2):1053-61
Linear ubiquitination prevents inflammation and regulates immune signalling.
Gerlach B, Cordier SM, Schmukle AC, Emmerich CH, Rieser E, Haas TL, Webb AI, Rickard JA, Anderton H, Wong WW, Nachbur U, Gangoda L, Warnken U, Purcell AW, Silke J, Walczak H
Nature 2011 Mar 31;471(7340):591-6
Nature 2011 Mar 31;471(7340):591-6
Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-κB pathway.
Jin HR, Jin X, Lee JJ
Biochemical and biophysical research communications 2010 Oct 1;400(4):581-6
Biochemical and biophysical research communications 2010 Oct 1;400(4):581-6
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse cIAP by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 or human cIAP-2. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse cIAP Pan-specific Monoclonal Antibody (Catalog # MAB3400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and SW3T3 mouse contact inhibited fibroblast cell line were included. Specific bands for cIAP-1 and cIAP-2 were detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human cIAP by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with either cIAP-1 or cIAP-2 and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP-1 and cIAP-2 at approximately 73 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse cIAP Pan-specific Monoclonal Antibody (Catalog # MAB3400). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.