MA5-14997

antibody from Invitrogen Antibodies

Targeting: BIRC3 API2, c-IAP2, cIAP2, hiap-1, MALT2, MIHC, RNF49

Immunoprecipitation

Antibody data

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Validation data

BIRC3 protein structure - MA5-14997 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of c-IAP2 in extracts from Raji and Ramos cell lines using c-IAP2 monoclonal antibody (Product # MA5-14997).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of c-IAP2 in extracts from Raji and Ramos cell lines using c-IAP2 monoclonal antibody (Product # MA5-14997).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of cIAP2 was achieved by transfecting Raji cells with cIAP2 specific siRNAs (Silencer® select Product # s1451, s1452). Western blot analysis (Fig. a) was performed using whole cell extracts from the cIAP2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with cIAP2 Monoclonal Antibody (Product # MA5-14997, 1:1000 dilution) and chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to cIAP2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-cIAP2 Monoclonal Antibody (Product # MA5-14997) and a 65 kDa band corresponding to cIAP2 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Raji (Lane 1) and Daudi (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).