PA5-19514

antibody from Invitrogen Antibodies

Targeting: BIRC3 API2, c-IAP2, cIAP2, hiap-1, MALT2, MIHC, RNF49

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19514

Provider

Invitrogen Antibodies

Product name

cIAP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

For Western Blot, this antibody has non-specific bands at 150 kDa,35 kDa and 75 kDa.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of a Whole Cell Lysate using Product # PA5-19514, cIAP2 primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-cIAP2 Polyclonal Antibody(Product # PA5-19514) and a 68kDa along with uncharacterized bands (*) corresponding to cIAP2 was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of Raji (Lane 1), Daudi (Lane 2), Jurkat (Lane 3), HeLa (Lane 4), IMR-32 (Lane 5), LNCaP (Lane 6) and tissue extract of Mouse Spleen (Lane 7), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody ( 1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using Product # PA5-19514, anti-cIAP2 antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).