PA5-29643

antibody from Invitrogen Antibodies

Targeting: BIRC3 API2, c-IAP2, cIAP2, hiap-1, MALT2, MIHC, RNF49

Antibody data

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Validation data

Product number: PA5-29643 - Provider product page
Provider: Invitrogen Antibodies
Product name: cIAP2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, HeLa WCE, HeLa cytosol, rat spleen.
Concentration: 0.67 mg/mL

BIRC3 protein structure - PA5-29643 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of cIAP2 using 30 µg of A) 293T (B) A431 (C) H1299 (D) HeLa (E) HepG2 and F) MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a cIAP2 polyclonal antibody (Product # PA5-29643) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of cIAP2 in HeLa whole cell extract, HeLa cytosol and HeLa nuclear extract (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with cIAP2 Polyclonal antibody (Product # PA5-29643) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of cIAP2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a cIAP2 Polyclonal Antibody (Product # PA5-29643) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of cIAP2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a cIAP2 Polyclonal Antibody (Product # PA5-29643) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: cIAP2 Polyclonal Antibody detects cIAP2 protein by western blot analysis. HeLa whole cell extract, HeLa cytosol and HeLa nuclear extract (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP2 Polyclonal Antibody (Product # PA5-29643) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: cIAP2 Polyclonal Antibody detects cIAP2 protein by western blot analysis. A. 50 µg rat spleen lysate/extract.7.5% SDS-PAGE. CIAP2 Polyclonal Antibody (Product # PA5-29643) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.