MA5-27498

antibody from Invitrogen Antibodies

Targeting: DPYSL2 CRMP2, DHPRP2, DRP-2, DRP2

Antibody data

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Validation data

DPYSL2 protein structure - MA5-27498 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DPYSL2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with DPYSL2 (Product # MA5-27498) monoclonal antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DPYSL2 in PC12, U87, NIH3T3 cells using 35 µg per lane. Samples were probed with DPYSL2 (Product # MA5-27498) monoclonal antibody at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CRMP2 was achieved by transfecting Neuro-2a with CRMP2 specific siRNAs (Silencer® select Product # s4269). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the CRMP2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CRMP2 Monoclonal Antibody (OTI5D7) (Product # MA5-27498, 1:2000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CRMP2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CRMP2 Monoclonal Antibody (OTI5D7), eBioscience™ (Product # MA5-27498) and a 60 kDa band corresponding to CRMP2 was observed across cell lines and tissues tested except MCF-7 and SK-BR-3 which are reported to be low protein expressing cell lines. Membrane enriched extracts (30 µg lysate) of PC-12 (Lane 1), Neuro-2a (Lane 2) and SH-SY5Y (Lane 3), U-87 MG (Lane 4), MCF-7 (Lane 5), MCF 10A (Lane 6), SK-BR-3 (Lane 7), tissue extracts of Mouse Brain (Lane 8) and Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).