LS-C745356

antibody from LSBio

Targeting: SPRY2 hSPRY2

Western blot

ELISA

Immunocytochemistry

Antibody data

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Validation data

Product number: LS-C745356 - Provider product page
Provider: LSBio
Product name: SPRY2 / Sprouty 2 Antibody (Internal) LS-C745356
Antibody type: Polyclonal
Description: Affinity purified
Reactivity: Human
Host: Rabbit
Isotype: IgG
Storage: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

SPRY2 protein structure - LS-C745356 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty2 antibody. Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4°C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty2 antibody. Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4°C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty-2 antibody. Tissue: liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-sprouty 2 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Sprouty-2 as precipitated red signal with hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemistry of rabbit anti-Sprouty2 antibody. Tissue: human kidney clear cell tumor. Fixation: formalin fixed, paraffin embedded. Antigen retrieval: yes. Primary antibody: Sprouty2 antibody at 1:250. Secondary antibody: alexafluor 488 secondary at 1:500 overnight at 4°C. Localization: nucleus and cytoplasm. Staining: green = sprouty2, Blue = dapi, red= vhl.