GTX50068

antibody from GeneTex

Targeting: CTSH ACC-4, ACC-5, ACC4, ACC5, CPSB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

GTX50068

Provider

GeneTex

Proper citation

GeneTex Cat#GTX50068, RRID:AB_11169065

Product name

Cathepsin H antibody [3G8]

Antibody type

Monoclonal

Antigen

Other

Description

By protein-G affinity chromatography

Reactivity

Human

Host

Mouse

Storage

Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

The recombinant proteins (Cathepsin B, D, H, k and S), and the extracts (40ug) of various tumor cell lines were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cathepsin H antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The upper arrow is a precathepsin H form, and the lower arrow is a mature form of cathepsin H. Lane 1: recombinant human Cathepsin B Lane 2: recombinant human Cathepsin D Lane 3: recombinant human Cathepsin H Lane 4: recombinant human Cathepsin K Lane 5: recombinant human Cathepsin S Lane 6: Ramos cell lysates Lane 7: Raji cell lysates Lane 8: U937 cell lysates Lane 9: HL-60 cell lysates Lane 10: U87MG cell lysates Lane 11: SH-SY5Y cell lysates Lane 12: HepG2 cell lysates Lane 13: KATO III cell lysates Lane 14: A549 cell lysates Lane 15: HT-29 cell lysates

Validation comment

WB