Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- SM6030S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM6030S, RRID:AB_1612477
- Product name
- anti Cathepsin H (23-335)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human Cathepsin H (aa 23-335) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G8
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The recombinant proteins (Cathepsin B, D, H, K and S), and the extracts (40 μg) of various tumor cell lines were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cathepsin H antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The upper arrow is a precathepsin H form, and the lower arrow is a mature form of cathepsin H.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The recombinant proteins (Cathepsin B, D, H, k and S), and the extracts (40ug) of various tumor cell lines were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human cathepsin H antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The upper arrow is a precathepsin H form, and the lower arrow is a mature form of cathepsin H.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SM6030 Cathepsin H antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SM6030 Cathepsin H antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.