NBP1-77533

antibody from Novus Biologicals

Targeting: TNFAIP3 A20, OTUD7C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-77533

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-77533, RRID:AB_11017330

Product name

Mouse Monoclonal A20/TNFAIP3 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

A20 Promotes Ripoptosome Formation and TNF-Induced Apoptosis via cIAPs Regulation and NIK Stabilization in Keratinocytes.

Feoktistova M, Makarov R, Brenji S, Schneider AT, Hooiveld GJ, Luedde T, Leverkus M, Yazdi AS, Panayotova-Dimitrova D
Cells 2020 Feb 3;9(2)

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Genome-wide analyses of long non-coding RNA expression profiles and functional network analysis in esophageal squamous cell carcinoma.

Ma J, Xiao Y, Tian B, Chen S, Zhang B, Wu J, Wu Z, Li X, Tang J, Yang D, Zhou Y, Wang H, Su M, Wang W
Scientific reports 2019 Jun 24;9(1):9162

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Pathogen-induced ubiquitin-editing enzyme A20 bifunctionally shuts off NF-κB and caspase-8-dependent apoptotic cell death.

Lim MCC, Maubach G, Sokolova O, Feige MH, Diezko R, Buchbinder J, Backert S, Schlüter D, Lavrik IN, Naumann M
Cell death and differentiation 2017 Sep;24(9):1621-1631

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Induction of the inflammatory regulator A20 by gibberellic acid in airway epithelial cells.

Reihill JA, Malcomson B, Bertelsen A, Cheung S, Czerwiec A, Barsden R, Elborn JS, Dürkop H, Hirsch B, Ennis M, Kelly C, Schock BC
British journal of pharmacology 2016 Feb;173(4):778-89

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Permissive expansion and homing of adoptively transferred T cells in tumor-bearing hosts.

Perez C, Jukica A, Listopad JJ, Anders K, Kühl AA, Loddenkemper C, Blankenstein T, Charo J
International journal of cancer 2015 Jul 15;137(2):359-71

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The deubiquitinase activity of A20 is dispensable for NF-κB signaling.

De A, Dainichi T, Rathinam CV, Ghosh S
EMBO reports 2014 Jul;15(7):775-83

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Transcription of Tnfaip3 is regulated by NF-κB and p38 via C/EBPβ in activated macrophages.

Lai TY, Wu SD, Tsai MH, Chuang EY, Chuang LL, Hsu LC, Lai LC
PloS one 2013;8(9):e73153

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Atlantic salmon type I IFN subtypes show differences in antiviral activity and cell-dependent expression: evidence for high IFNb/IFNc-producing cells in fish lymphoid tissues.

Svingerud T, Solstad T, Sun B, Nyrud ML, Kileng Ø, Greiner-Tollersrud L, Robertsen B
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5912-23

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Identification of FOXO3 and PRDM1 as tumor-suppressor gene candidates in NK-cell neoplasms by genomic and functional analyses.

Karube K, Nakagawa M, Tsuzuki S, Takeuchi I, Honma K, Nakashima Y, Shimizu N, Ko YH, Morishima Y, Ohshima K, Nakamura S, Seto M
Blood 2011 Sep 22;118(12):3195-204

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Sustained NF-kappaB activity in chronic lymphocytic leukemia is independent of genetic and epigenetic alterations in the TNFAIP3 (A20) locus.

Frenzel LP, Claus R, Plume N, Schwamb J, Konermann C, Pallasch CP, Claasen J, Brinker R, Wollnik B, Plass C, Wendtner CM
International journal of cancer 2011 May 15;128(10):2495-500

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Acquisition of host-derived CD40L by HIV-1 in vivo and its functional consequences in the B-cell compartment.

Imbeault M, Ouellet M, Giguère K, Bertin J, Bélanger D, Martin G, Tremblay MJ
Journal of virology 2011 Mar;85(5):2189-200

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Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus.

Adrianto I, Wen F, Templeton A, Wiley G, King JB, Lessard CJ, Bates JS, Hu Y, Kelly JA, Kaufman KM, Guthridge JM, Alarcón-Riquelme ME, BIOLUPUS and GENLES Networks., Anaya JM, Bae SC, Bang SY, Boackle SA, Brown EE, Petri MA, Gallant C, Ramsey-Goldman R, Reveille JD, Vila LM, Criswell LA, Edberg JC, Freedman BI, Gregersen PK, Gilkeson GS, Jacob CO, James JA, Kamen DL, Kimberly RP, Martin J, Merrill JT, Niewold TB, Park SY, Pons-Estel BA, Scofield RH, Stevens AM, Tsao BP, Vyse TJ, Langefeld CD, Harley JB, Moser KL, Webb CF, Humphrey MB, Montgomery CG, Gaffney PM
Nature genetics 2011 Mar;43(3):253-8

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A20 deficiency in B cells enhances B-cell proliferation and results in the development of autoantibodies.

Hövelmeyer N, Reissig S, Xuan NT, Adams-Quack P, Lukas D, Nikolaev A, Schlüter D, Waisman A
European journal of immunology 2011 Mar;41(3):595-601

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Linear ubiquitination prevents inflammation and regulates immune signalling.

Gerlach B, Cordier SM, Schmukle AC, Emmerich CH, Rieser E, Haas TL, Webb AI, Rickard JA, Anderton H, Wong WW, Nachbur U, Gangoda L, Warnken U, Purcell AW, Silke J, Walczak H
Nature 2011 Mar 31;471(7340):591-6

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Cleavage by MALT1 induces cytosolic release of A20.

Malinverni C, Unterreiner A, Staal J, Demeyer A, Galaup M, Luyten M, Beyaert R, Bornancin F
Biochemical and biophysical research communications 2010 Oct 1;400(4):543-7

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Cell-permeable peptide DEPDC1-ZNF224 interferes with transcriptional repression and oncogenicity in bladder cancer cells.

Harada Y, Kanehira M, Fujisawa Y, Takata R, Shuin T, Miki T, Fujioka T, Nakamura Y, Katagiri T
Cancer research 2010 Jul 15;70(14):5829-39

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Targeting A20 decreases glioma stem cell survival and tumor growth.

Hjelmeland AB, Wu Q, Wickman S, Eyler C, Heddleston J, Shi Q, Lathia JD, Macswords J, Lee J, McLendon RE, Rich JN
PLoS biology 2010 Feb 23;8(2):e1000319

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Cellular expression of A20 and ABIN-3 in response to Toll-like receptor-4 stimulation.

Verhelst K, Verstrepen L, Coornaert B, Carpentier I, Beyaert R
Methods in molecular biology (Clifton, N.J.) 2009;517:205-15

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TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkin lymphomas.

Honma K, Tsuzuki S, Nakagawa M, Tagawa H, Nakamura S, Morishima Y, Seto M
Blood 2009 Sep 17;114(12):2467-75

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Prognostic impact of O6-methylguanine-DNA methyltransferase silencing in patients with recurrent glioblastoma multiforme who undergo surgery and carmustine wafer implantation: a prospective patient cohort.

Metellus P, Coulibaly B, Nanni I, Fina F, Eudes N, Giorgi R, Barrie M, Chinot O, Fuentes S, Dufour H, Ouafik L, Figarella-Branger D
Cancer 2009 Oct 15;115(20):4783-94

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Essential role of MALT1 protease activity in activated B cell-like diffuse large B-cell lymphoma.

Hailfinger S, Lenz G, Ngo V, Posvitz-Fejfar A, Rebeaud F, Guzzardi M, Penas EM, Dierlamm J, Chan WC, Staudt LM, Thome M
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 24;106(47):19946-51

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TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma.

Schmitz R, Hansmann ML, Bohle V, Martin-Subero JI, Hartmann S, Mechtersheimer G, Klapper W, Vater I, Giefing M, Gesk S, Stanelle J, Siebert R, Küppers R
The Journal of experimental medicine 2009 May 11;206(5):981-9

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A20 negatively regulates T cell receptor signaling to NF-kappaB by cleaving Malt1 ubiquitin chains.

Düwel M, Welteke V, Oeckinghaus A, Baens M, Kloo B, Ferch U, Darnay BG, Ruland J, Marynen P, Krappmann D
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7718-28

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Attenuated expression of A20 markedly increases the efficacy of double-stranded RNA-activated dendritic cells as an anti-cancer vaccine.

Breckpot K, Aerts-Toegaert C, Heirman C, Peeters U, Beyaert R, Aerts JL, Thielemans K
Journal of immunology (Baltimore, Md. : 1950) 2009 Jan 15;182(2):860-70

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The zinc finger protein A20 targets TRAF2 to the lysosomes for degradation.

Li L, Soetandyo N, Wang Q, Ye Y
Biochimica et biophysica acta 2009 Feb;1793(2):346-53

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Involvement of Ymer in suppression of NF-kappaB activation by regulated interaction with lysine-63-linked polyubiquitin chain.

Bohgaki M, Tsukiyama T, Nakajima A, Maruyama S, Watanabe M, Koike T, Hatakeyama S
Biochimica et biophysica acta 2008 May;1783(5):826-37

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T cell antigen receptor stimulation induces MALT1 paracaspase-mediated cleavage of the NF-kappaB inhibitor A20.

Coornaert B, Baens M, Heyninck K, Bekaert T, Haegman M, Staal J, Sun L, Chen ZJ, Marynen P, Beyaert R
Nature immunology 2008 Mar;9(3):263-71

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Localization of A20 to a lysosome-associated compartment and its role in NFkappaB signaling.

Li L, Hailey DW, Soetandyo N, Li W, Lippincott-Schwartz J, Shu HB, Ye Y
Biochimica et biophysica acta 2008 Jun;1783(6):1140-9

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Measles virus P protein suppresses Toll-like receptor signal through up-regulation of ubiquitin-modifying enzyme A20.

Yokota S, Okabayashi T, Yokosawa N, Fujii N
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jan;22(1):74-83

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Encoding NF-kappaB temporal control in response to TNF: distinct roles for the negative regulators IkappaBalpha and A20.

Werner SL, Kearns JD, Zadorozhnaya V, Lynch C, O'Dea E, Boldin MP, Ma A, Baltimore D, Hoffmann A
Genes & development 2008 Aug 1;22(15):2093-101

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A20 inhibits human salivary adenoid cystic carcinoma cells invasion via blocking nuclear factor-kappaB activation.

Zhang B, Guan CC, Chen WT, Zhang P, Yan M, Shi JH, Qin CL, Yang Q
Chinese medical journal 2007 Oct 20;120(20):1830-5

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IkappaB kinase beta phosphorylates the K63 deubiquitinase A20 to cause feedback inhibition of the NF-kappaB pathway.

Hutti JE, Turk BE, Asara JM, Ma A, Cantley LC, Abbott DW
Molecular and cellular biology 2007 Nov;27(21):7451-61

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Identification of interleukin-1beta regulated genes in uterine smooth muscle cells.

Chevillard G, Derjuga A, Devost D, Zingg HH, Blank V
Reproduction (Cambridge, England) 2007 Dec;134(6):811-22

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Toll-like receptor 3 expressed by melanoma cells as a target for therapy?

Salaun B, Lebecque S, Matikainen S, Rimoldi D, Romero P
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Aug 1;13(15 Pt 1):4565-74

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A20 is a potent inhibitor of TLR3- and Sendai virus-induced activation of NF-kappaB and ISRE and IFN-beta promoter.

Wang YY, Li L, Han KJ, Zhai Z, Shu HB
FEBS letters 2004 Oct 8;576(1-2):86-90

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Cytoprotective and antiapoptotic effects of IL-13 in hepatic cold ischemia/reperfusion injury are heme oxygenase-1 dependent.

Ke B, Shen XD, Lassman CR, Gao F, Busuttil RW, Kupiec-Weglinski JW
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2003 Sep;3(9):1076-82

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Involvement of phosphatidylinositol 3-kinase gamma in neutrophil apoptosis.

Yang KY, Arcaroli J, Kupfner J, Pitts TM, Park JS, Strasshiem D, Perng RP, Abraham E
Cellular signalling 2003 Feb;15(2):225-33

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Heme oxygenase 1 mediates the immunomodulatory and antiapoptotic effects of interleukin 13 gene therapy in vivo and in vitro.

Ke B, Shen XD, Zhai Y, Gao F, Busuttil RW, Volk HD, Kupiec-Weglinski JW
Human gene therapy 2002 Oct 10;13(15):1845-57

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The promyelocytic leukemia protein represses A20-mediated transcription.

Wu WS, Xu ZX, Chang KS
The Journal of biological chemistry 2002 Aug 30;277(35):31734-9

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Role of NF-kappaB in endotoxemia-induced alterations of lung neutrophil apoptosis.

Kupfner JG, Arcaroli JJ, Yum HK, Nadler SG, Yang KY, Abraham E
Journal of immunology (Baltimore, Md. : 1950) 2001 Dec 15;167(12):7044-51

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Lane view shows a specific band for A20/TNFAIP3 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of A20 using mAb 59A426. A20 migrated as a stack of bands and LPS treatment (200 ng, ) induced a ~58 kDa putative A20 cleavage band* not seen in the untreated control (C) THP1 cells. A20 cleavage fragments have often been described to accompany NF-kB activation (see Product Citations). Although the significance of the cleavage fragments remains to be fully elucidated, they perk interest because cleavage fragments often have key roles in signaling pathways. Densitometry analysis performed by Shayna Stein in ImageJ.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human Jurkat lysate probed with A20 antibody at 4 ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Human placenta probed with A20 antibody at 5 ug/ml, cytoplasmic staining of decidual cells is observed. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Intracellular flow cytometric analysis of A20 in ThP-1 cells using A20 antibody (red) and isotype control (green) at 0.5 ug/ml

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on Daudi cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - Analysis of PE conjugate of NBP1-77533. An intracellular stain was performed on Jurkat cells with A20/TNFAIP3 antibody (59A426) NBP1-77533PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized wit

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on SK-MEL-28 cells with A20/TNFAIP3 (59A426) antibody NBP1-77533PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on SK-MEL-28 cells with A20/TNFAIP3 (59A426) antibody NBP1-77533AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on HeLa cells with A20/TNFAIP3 (59A426) antibody NBP1-77533F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: A20/TNFAIP3 Antibody (59A426) [NBP1-77533] - An intracellular stain was performed on RH-30 cells with A20/TNFAIP3 Antibody (59A426) NBP1-77533PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.