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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Other assay [2]
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- Product number
- MA1-12297 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin D2 Monoclonal Antibody (DCS3.1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- DCS3.1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Functional adenosine triphosphate-sensitive potassium channel is required in high-carbohydrate diet-induced increase in β-cell mass.
Sustained expression of the transcription factor GLIS3 is required for normal beta cell function in adults.
GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1).
GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1).
Cyclin D3 compensates for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression.
Cyclin D2 protein stability is regulated in pancreatic beta-cells.
Control of cyclin D1 and breast tumorigenesis by the EglN2 prolyl hydroxylase.
Adaptive beta-cell proliferation is severely restricted with advanced age.
Functions of p21 and p27 in the regenerating epithelial linings of the mouse small and large intestine.
Functions of p21 and p27 in the regenerating epithelial linings of the mouse small and large intestine.
Molecular characterization of spontaneous mesenchymal stem cell transformation.
Selective cortical interneuron and GABA deficits in cyclin D2-null mice.
Effects of granulosa cell-specific deletion of Rb in Inha-alpha null female mice.
Loss of cyclin D1 impairs cerebellar development and suppresses medulloblastoma formation.
p75NTR enhances PC12 cell tumor growth by a non-receptor mechanism involving downregulation of cyclin D2.
Pomegranate fruit juice for chemoprevention and chemotherapy of prostate cancer.
Molecular mechanisms for apigenin-induced cell-cycle arrest and apoptosis of hormone refractory human prostate carcinoma DU145 cells.
Cdk4 is indispensable for postnatal proliferation of the anterior pituitary.
Cyclin D2 compensates for the loss of cyclin D1 in estrogen-induced mouse uterine epithelial cell proliferation.
Molecular pathway for (-)-epigallocatechin-3-gallate-induced cell cycle arrest and apoptosis of human prostate carcinoma cells.
Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells.
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
Regulation of exit from quiescence by p27 and cyclin D1-CDK4.
Regulation of exit from quiescence by p27 and cyclin D1-CDK4.
Murase M, Seino Y, Maekawa R, Iida A, Hosokawa K, Hayami T, Tsunekawa S, Hamada Y, Yokoi N, Seino S, Hayashi Y, Arima H
Journal of diabetes investigation 2019 Mar;10(2):238-250
Journal of diabetes investigation 2019 Mar;10(2):238-250
Sustained expression of the transcription factor GLIS3 is required for normal beta cell function in adults.
Yang Y, Chang BH, Chan L
EMBO molecular medicine 2013 Jan;5(1):92-104
EMBO molecular medicine 2013 Jan;5(1):92-104
GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1).
Stein J, Milewski WM, Hara M, Steiner DF, Dey A
Islets 2011 Jan-Feb;3(1):21-34
Islets 2011 Jan-Feb;3(1):21-34
GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1).
Stein J, Milewski WM, Hara M, Steiner DF, Dey A
Islets 2011 Jan-Feb;3(1):21-34
Islets 2011 Jan-Feb;3(1):21-34
Cyclin D3 compensates for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression.
Zhang Q, Sakamoto K, Liu C, Triplett AA, Lin WC, Rui H, Wagner KU
Cancer research 2011 Dec 15;71(24):7513-24
Cancer research 2011 Dec 15;71(24):7513-24
Cyclin D2 protein stability is regulated in pancreatic beta-cells.
He LM, Sartori DJ, Teta M, Opare-Addo LM, Rankin MM, Long SY, Diehl JA, Kushner JA
Molecular endocrinology (Baltimore, Md.) 2009 Nov;23(11):1865-75
Molecular endocrinology (Baltimore, Md.) 2009 Nov;23(11):1865-75
Control of cyclin D1 and breast tumorigenesis by the EglN2 prolyl hydroxylase.
Zhang Q, Gu J, Li L, Liu J, Luo B, Cheung HW, Boehm JS, Ni M, Geisen C, Root DE, Polyak K, Brown M, Richardson AL, Hahn WC, Kaelin WG Jr, Bommi-Reddy A
Cancer cell 2009 Nov 6;16(5):413-24
Cancer cell 2009 Nov 6;16(5):413-24
Adaptive beta-cell proliferation is severely restricted with advanced age.
Rankin MM, Kushner JA
Diabetes 2009 Jun;58(6):1365-72
Diabetes 2009 Jun;58(6):1365-72
Functions of p21 and p27 in the regenerating epithelial linings of the mouse small and large intestine.
Zheng Y, Bie W, Yang R, Perekatt AO, Poole AJ, Tyner AL
Cancer biology & therapy 2008 Jun;7(6):873-9
Cancer biology & therapy 2008 Jun;7(6):873-9
Functions of p21 and p27 in the regenerating epithelial linings of the mouse small and large intestine.
Zheng Y, Bie W, Yang R, Perekatt AO, Poole AJ, Tyner AL
Cancer biology & therapy 2008 Jun;7(6):873-9
Cancer biology & therapy 2008 Jun;7(6):873-9
Molecular characterization of spontaneous mesenchymal stem cell transformation.
Rubio D, Garcia S, Paz MF, De la Cueva T, Lopez-Fernandez LA, Lloyd AC, Garcia-Castro J, Bernad A
PloS one 2008 Jan 2;3(1):e1398
PloS one 2008 Jan 2;3(1):e1398
Selective cortical interneuron and GABA deficits in cyclin D2-null mice.
Glickstein SB, Moore H, Slowinska B, Racchumi J, Suh M, Chuhma N, Ross ME
Development (Cambridge, England) 2007 Nov;134(22):4083-93
Development (Cambridge, England) 2007 Nov;134(22):4083-93
Effects of granulosa cell-specific deletion of Rb in Inha-alpha null female mice.
Andreu-Vieyra C, Chen R, Matzuk MM
Endocrinology 2007 Aug;148(8):3837-49
Endocrinology 2007 Aug;148(8):3837-49
Loss of cyclin D1 impairs cerebellar development and suppresses medulloblastoma formation.
Pogoriler J, Millen K, Utset M, Du W
Development (Cambridge, England) 2006 Oct;133(19):3929-37
Development (Cambridge, England) 2006 Oct;133(19):3929-37
p75NTR enhances PC12 cell tumor growth by a non-receptor mechanism involving downregulation of cyclin D2.
Fritz MD, Mirnics ZK, Nylander KD, Schor NF
Experimental cell research 2006 Oct 15;312(17):3287-97
Experimental cell research 2006 Oct 15;312(17):3287-97
Pomegranate fruit juice for chemoprevention and chemotherapy of prostate cancer.
Malik A, Afaq F, Sarfaraz S, Adhami VM, Syed DN, Mukhtar H
Proceedings of the National Academy of Sciences of the United States of America 2005 Oct 11;102(41):14813-8
Proceedings of the National Academy of Sciences of the United States of America 2005 Oct 11;102(41):14813-8
Molecular mechanisms for apigenin-induced cell-cycle arrest and apoptosis of hormone refractory human prostate carcinoma DU145 cells.
Shukla S, Gupta S
Molecular carcinogenesis 2004 Feb;39(2):114-26
Molecular carcinogenesis 2004 Feb;39(2):114-26
Cdk4 is indispensable for postnatal proliferation of the anterior pituitary.
Jirawatnotai S, Aziyu A, Osmundson EC, Moons DS, Zou X, Kineman RD, Kiyokawa H
The Journal of biological chemistry 2004 Dec 3;279(49):51100-6
The Journal of biological chemistry 2004 Dec 3;279(49):51100-6
Cyclin D2 compensates for the loss of cyclin D1 in estrogen-induced mouse uterine epithelial cell proliferation.
Chen B, Pollard JW
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1368-81
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1368-81
Molecular pathway for (-)-epigallocatechin-3-gallate-induced cell cycle arrest and apoptosis of human prostate carcinoma cells.
Gupta S, Hussain T, Mukhtar H
Archives of biochemistry and biophysics 2003 Feb 1;410(1):177-85
Archives of biochemistry and biophysics 2003 Feb 1;410(1):177-85
Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells.
Gupta S, Afaq F, Mukhtar H
Oncogene 2002 May 23;21(23):3727-38
Oncogene 2002 May 23;21(23):3727-38
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.
Clark W, Black EJ, MacLaren A, Kruse U, LaThangue N, Vogt PK, Gillespie DA
Molecular and cellular biology 2000 Apr;20(7):2529-42
Molecular and cellular biology 2000 Apr;20(7):2529-42
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.
Clark W, Black EJ, MacLaren A, Kruse U, LaThangue N, Vogt PK, Gillespie DA
Molecular and cellular biology 2000 Apr;20(7):2529-42
Molecular and cellular biology 2000 Apr;20(7):2529-42
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
Tsutsui T, Hesabi B, Moons DS, Pandolfi PP, Hansel KS, Koff A, Kiyokawa H
Molecular and cellular biology 1999 Oct;19(10):7011-9
Molecular and cellular biology 1999 Oct;19(10):7011-9
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
Tsutsui T, Hesabi B, Moons DS, Pandolfi PP, Hansel KS, Koff A, Kiyokawa H
Molecular and cellular biology 1999 Oct;19(10):7011-9
Molecular and cellular biology 1999 Oct;19(10):7011-9
Regulation of exit from quiescence by p27 and cyclin D1-CDK4.
Ladha MH, Lee KY, Upton TM, Reed MF, Ewen ME
Molecular and cellular biology 1998 Nov;18(11):6605-15
Molecular and cellular biology 1998 Nov;18(11):6605-15
Regulation of exit from quiescence by p27 and cyclin D1-CDK4.
Ladha MH, Lee KY, Upton TM, Reed MF, Ewen ME
Molecular and cellular biology 1998 Nov;18(11):6605-15
Molecular and cellular biology 1998 Nov;18(11):6605-15
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Insulin secretion and change in messenger ribonucleic acid expression levels in MIN 6-K8 beta-cells. (a) Effects of glucose, glimepiride and nifedipine on insulin secretion in MIN 6-K8 beta-cells. Insulin secretion from MIN 6-K8 beta-cells was normalized by cellular insulin content ( n = 7 per group). (b, c) Messenger ribonucleic acid expression levels of cyclinD2 and Irs2 in MIN 6-K8 beta-cells ( n = 9 per group). (d) Representative immunoblots and (e) relative cyclin D2 protein expression levels normalized with respect to those of actin in MIN 6-K8 beta-cells ( n = 12 per group). (f) Effect of S961 on insulin secretion in MIN 6-K8 beta-cells. Insulin secretion from MIN 6-K8 beta-cells was normalized by cellular insulin content ( n = 12 per group). (g, h) Messenger ribonucleic acid expression levels of cyclinD2 and Irs2 in MIN 6-K8 beta-cells ( n = 11-12; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data are expressed as means +- standard error of the mean. CCB , nifedipine; HG , 16.7 mmol/L glucose; LG , 2.8 mmol/L glucose; SU , glimepiride.
