PA5-90889

antibody from Invitrogen Antibodies

Targeting: LAMC1 LAMB2

ELISA

Antibody data

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Validation data

Product number: PA5-90889 - Provider product page
Provider: Invitrogen Antibodies
Product name: Laminin gamma-1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Positive Samples: LO2, Mouse heart, Mouse kidney, Rat heart Immunogen sequence: ECPACYRLVK DKVADHRVKL QELESLIANL GTGDEMVTDQ AFEDRLKEAE REVMDLLREA QDVKDVDQNL MDRLQRVNNT LSSQISRLQN IRNTIEETGN LAEQARAHVE NTERLIEIAS RELEKAKVAA ANVSVTQPES TGDPNNMTLL AEEARKLAER HKQEADDIVR VAKTANDTST EAYNLLLRTL AGENQTAFEI EELNRKYEQA KNISQDLEKQ AARVHEEAKR AGDKAVEIYA SVAQLS
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.84 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles

LAMC1 protein structure - PA5-90889 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Laminin gamma-1 in extracts of various cell lines. Samples were incubated with Laminin gamma-1 Polyclonal antibody (Product # PA5-90889) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Laminin gamma-1 in extracts of LO2 cells. Samples were incubated with Laminin gamma-1 Polyclonal antibody (Product # PA5-90889) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of LAMC1 was achieved by transfecting Hep G2 with LAMC1 specific siRNAs (Silencer® select Product # S8078, S8079). Western blot analysis (Fig. a) was performed using Whole cell extracts from the LAMC1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Laminin gamma-1 Polyclonal Antibody (Product # PA5-90889, 1:2,500) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to LAMC1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Laminin gamma-1 Polyclonal Antibody (Product # PA5-90889) and a 210 kDa band corresponding to LAMC1 was observed across the panel except for K-562 (Fig. a) and mouse liver and rat liver (Fig. b) which are reported to be negative expressors of LAMC1. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), A-431 (Lane 2), BeWo (Lane 3), PC-3 (Lane 4), DU 145 (Lane 5), K-562 (Lane 6) (Fig. a) and tissue extracts (30 µg lysate) of Mouse Lung (Lane 1), Mouse Liver (Lane 2), Rat Lung (Lane 3), Rat Liver (Lane 4) (Fig. b) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2,500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Laminin gamma-1 in paraffin-embedded human breast cancer using. Samples were incubated with Laminin gamma-1 Polyclonal antibody (Product # PA5-90889) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Laminin gamma-1 in paraffin-embedded human uterus. Samples were incubated with Laminin gamma-1 Polyclonal antibody (Product # PA5-90889) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Laminin gamma-1 in paraffin-embedded human uterine cancer. Samples were incubated with Laminin gamma-1 Polyclonal antibody (Product # PA5-90889) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Laminin gamma-1 in paraffin-embedded human breast cancer using. Samples were incubated with Laminin gamma-1 Polyclonal antibody (Product # PA5-90889) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Laminin gamma-1 in paraffin-embedded human uterus. Samples were incubated with Laminin gamma-1 Polyclonal antibody (Product # PA5-90889) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.