Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-51136 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF2BP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous levels of total IGF2BP3 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.9 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGF2BP3 was performed by loading Hela cell lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a IGF2BP3 antibody (Product # PA5-51136) at a 1/200 dilution for 10 seconds, followed by Goat anti rabbit IgG at 1/8000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IGF2BP3 in paraffin embedded Human lung cancer tissue using IGF2BP3 Polyclonal Antibody (Product # PA5-51136) at a 1:25 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RNA Immunoprecipitation (RIP) assay of endogenous IGF2BP3 protein using Anti-IGF2BP3 Antibody: RIP assay was performed using Anti-IGF2BP3 Polyclonal Antibody (Product # PA5-51136) 5 µg, on whole cell lysate from Hep G2 cells. Normal Rabbit IgG was used as a negative IP control. RNA purified by RiboPure™ RNA Purification Kit (Product # AM1924) was analyzed by RT-PCR using the Power SYBR® Green RNA-to-CT™ 1-Step Kit (Product # 4389986) with the primers pairs over MYC exon1, IGF2 and 18S rRNA. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.