Explore
Validate
Learn
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-96288 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF2BP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: HepG2, A-549, 293T, HeLa; Cellular Location: Cytoplasm, Nucleus Immunogen sequence: KKIEQDTDTK ITISPLQELT LYNPERTITV KGNVETCAKA EEEIMKKIRE SYENDIASMN LQAHLIPGLN LNALGLFPPT SGMPPPTSGP PSAMTPPYPQ FEQSETETVH LFIPALSVGA IIGKQGQHIK QLSRFAGASI KIAPAEAPDA KVRMVIITGP PEAQFKAQGR IYGKIKEENF VSPKEEVKLE AHIRVPSFAA GRVIGKGGKT VNELQNLSSA EVVVPRDQTP DENDQVVVKI TGHFYACQVA QRKIQEILTQ VKQHQQQKAL QSGPPQSRRK
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.01 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IGF2BP3 was performed in U2OS cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96288).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of IGF2BP3 in U2OS cells. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96288) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of IGF2BP3 in 100 μg extracts of HepG2 cells. Samples were precipitated with 3 μg IGF2BP3 Polyclonal antibody (Product # PA5-96288). Western blot was performed from the immunoprecipitate using IGF2BP3 Polyclonal antibody (Product # PA5-96288) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded human esophagus. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96288) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded human stomach. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96288) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded mouse lung. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96288) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of IGF2BP3 was performed in 100 µg extracts of HepG2 cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96288). Western blot was performed from the immunoprecipitate using IGF2BP3 Polyclonal Antibody.
