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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-96288 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF2BP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: HepG2, A-549, 293T, HeLa; Cellular Location: Cytoplasm, Nucleus Immunogen sequence: KKIEQDTDTK ITISPLQELT LYNPERTITV KGNVETCAKA EEEIMKKIRE SYENDIASMN LQAHLIPGLN LNALGLFPPT SGMPPPTSGP PSAMTPPYPQ FEQSETETVH LFIPALSVGA IIGKQGQHIK QLSRFAGASI KIAPAEAPDA KVRMVIITGP PEAQFKAQGR IYGKIKEENF VSPKEEVKLE AHIRVPSFAA GRVIGKGGKT VNELQNLSSA EVVVPRDQTP DENDQVVVKI TGHFYACQVA QRKIQEILTQ VKQHQQQKAL QSGPPQSRRK
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.01 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IGF2BP3 in extracts of various cell lines using IGF2BP3 Polyclonal Antibody (Product # PA5-96288) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IGF2BP3 was performed in U2OS cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96288).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of IGF2BP3 was performed in 100 µg extracts of HepG2 cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96288). Western blot was performed from the immunoprecipitate using IGF2BP3 Polyclonal Antibody.
