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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-96506 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF2BP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KKIEQDTDTK ITISPLQELT LYNPERTITV KGNVETCAKA EEEIMKKIRE SYENDIASMN LQAHLIPGLN LNALGLFPPT SGMPPPTSGP PSAMTPPYPQ FEQSETETVH LFIPALSVGA IIGKQGQHIK QLSRFAGASI KIAPAEAPDA KVRMVIITGP PEAQFKAQGR IYGKIKEENF VSPKEEVKLE AHIRVPSFAA GRVIGKGGKT VNELQNLSSA EVVVPRDQTP DENDQVVVKI TGHFYACQVA QRKIQEILTQ VKQHQQQKAL QSGPPQSRRK; Positive Samples: HepG2, A-549, 293T, HeLa, Mouse testis; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.12 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGF2BP3 in rat testis. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IGF2BP3 in various lysates. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IGF2BP3 was performed in L929 cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96506).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of IGF2BP3 in NIH/3T3 cells. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of IGF2BP3 in 100 μg extracts of HepG2 cells. Samples were precipitated with 3 μg IGF2BP3 Polyclonal antibody (Product # PA5-96506). Western blot was performed from the immunoprecipitate using IGF2BP3 Polyclonal antibody (Product # PA5-96506) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded rat testis. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded mouse testis. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded human liver. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded mouse testis. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IGF2BP3 in paraffin-embedded human liver. Samples were incubated with IGF2BP3 Polyclonal antibody (Product # PA5-96506) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of IGF2BP3 was performed in 100 µg extracts of HepG2 cells using IGF2BP3 Polyclonal Antibody (Product # PA5-96506). Western blot was performed from the immunoprecipitate using IGF2BP3 Polyclonal Antibody.
