Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IGF2BP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: PC-3, SK-N-SH. Predicted reactivity: Mouse (98%), Zebrafish (80%), Xenopus laevis (83%), Chicken (82%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.56 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Diagnostic value of IMP3 and mesothelin in differentiating pancreatic ductal adenocarcinoma from chronic pancreatitis.
Ibrahim DA, Abouhashem NS
Pathology, research and practice 2016 Apr;212(4):288-93
Pathology, research and practice 2016 Apr;212(4):288-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IGF2BP3 Polyclonal Antibody (Product # PA5-30398). Sample (30 µg of whole cell lysate). Lane A: PC-3. Lane B: SK-N-SH . 7.5% SDS PAGE. IGF2BP3 Polyclonal Antibody (Product # PA5-30398) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IGF2BP3 Polyclonal Antibody detects IMP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IMP3 protein stained by IGF2BP3 Polyclonal Antibody (Product # PA5-30398) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IMP-3 (Product # PA5-30398) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.